Department of Chemical Engineering, The Johns Hopkins University, Baltimore, Maryland 21218, and Center of Marine Biotechnology, University of Maryland, Baltimore, Maryland 21202.
Appl Environ Microbiol. 1992 Apr;58(4):1134-41. doi: 10.1128/aem.58.4.1134-1141.1992.
Pyrococcus furiosus was shown to grow on casein or peptides as the sole carbon, energy, and nitrogen sources, while maltose could be used as a carbon and energy source only if peptides were present in the medium. A mixture of all 20 single amino acids could not replace the peptide requirement. Specific intracellular proteolytic activity was induced under low casein or tryptone levels and was decreased by the addition of maltose to both peptide-limiting and peptide-rich media in batch and continuous cultures. In a peptide-limited chemostat, activity towards azocasein and MeO-Suc-Arg-Pro-Tyr-p-nitroanilide reached a maximum at a dilution rate of 0.28 h, while activity toward l-lysine-p-nitroanilide reached a maximum at 0.50 h. Under peptide-limiting conditions, levels of the 66-kDa protease (S66) were enhanced relative to those of other cell proteins. Preliminary evidence suggests that this protease is immunologically related to the eukaryotic multicatalytic proteinase complex (proteosome).
Pyrococcus furiosus 被证明可以利用酪蛋白或肽作为唯一的碳源、能源和氮源进行生长，而麦芽糖只能在培养基中存在肽时作为碳源和能源使用。所有 20 种单一氨基酸的混合物不能替代肽的需求。在低酪蛋白或色氨酸水平下，会诱导出特定的细胞内蛋白水解活性，并且在分批和连续培养中，向肽限制和肽丰富的培养基中添加麦芽糖会降低这种活性。在肽限制的恒化器中，对偶氮酪蛋白和 MeO-Suc-Arg-Pro-Tyr-p-硝基苯胺的活性在稀释率为 0.28 h 时达到最大值，而对 l-赖氨酸-p-硝基苯胺的活性在 0.50 h 时达到最大值。在肽限制条件下，66 kDa 蛋白酶 (S66) 的水平相对于其他细胞蛋白增强。初步证据表明，这种蛋白酶与真核多催化蛋白酶复合物（蛋白酶体）在免疫学上有关。
