Kacharmina J E, Job C, Crino P, Eberwine J
Departments of Pharmacology and Psychiatry, and Neurology, University of Pennsylvania Medical Center, Philadelphia, PA 19104-6084, USA.
Proc Natl Acad Sci U S A. 2000 Oct 10;97(21):11545-50. doi: 10.1073/pnas.97.21.11545.
The selective subcellular localization of mRNAs to dendrites and the recent demonstration of local protein synthesis have highlighted the potential role of postsynaptic sites in modulation of cell-cell communication. We show that epitope-tagged subunit 2 of the ionotopic glutamate receptor, GluR2, mRNA transfected into isolated hippocampal neuronal dendrites is translated in response to pharmacologic stimulation. Further, confocal imaging of N-terminally labeled GluR2 reveals that the newly synthesized GluR2 protein can integrate into the dendritic membrane with the N terminus externally localized. These data demonstrate that integral membrane proteins can be synthesized in dendrites and can locally integrate into the cell membrane.
mRNA在树突中的选择性亚细胞定位以及近期关于局部蛋白质合成的证明，突出了突触后位点在调节细胞间通讯中的潜在作用。我们发现，转染到分离的海马神经元树突中的离子型谷氨酸受体GluR2的表位标记亚基2 mRNA，会响应药理刺激而被翻译。此外，对N端标记的GluR2进行共聚焦成像显示，新合成的GluR2蛋白能够整合到树突膜中，且N端位于细胞外部。这些数据表明，整合膜蛋白能够在树突中合成，并能在局部整合到细胞膜中。
