Hanton SD, Liu XM
Air Products and Chemicals, Inc., Allentown, Pennsylvania 18195, USA.
Anal Chem. 2000 Oct 1;72(19):4550-4. doi: 10.1021/ac000095n.
Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry is an important technique to characterize the average molecular weights, oligomer repeat units, and end groups of polymer materials. Although MALDI methods have been developed for a broad variety of different synthetic polymers, MALDI continues to struggle with polymer samples having broad polydispersity (PD). We have combined MALDI and gel permeation chromatography (GPC) analyses for broad PD polymer samples with the use of a liquid chromatography (LC) interface. The LC interface uses heated sheath gas and a capillary nozzle to remove most of the mobile phase and deposit the GPC eluants on the precoated matrix on a moving MALDI plate. Our experiments demonstrate that the combination of GPC-LC interface-MALDI can aid in the characterization of broad PD samples, the verification of the presence of low-intensity, high-mass oligomers, and the detection of minor series in polymer samples.
基质辅助激光解吸/电离（MALDI）质谱分析法是一种用于表征聚合物材料的平均分子量、低聚物重复单元和端基的重要技术。尽管已经针对多种不同的合成聚合物开发了MALDI方法，但MALDI对于具有宽多分散性（PD）的聚合物样品仍存在困难。我们通过使用液相色谱（LC）接口，将MALDI和凝胶渗透色谱（GPC）分析相结合，用于分析宽PD聚合物样品。该LC接口使用加热的鞘气和毛细管喷嘴去除大部分流动相，并将GPC洗脱液沉积在移动的MALDI板上预涂覆的基质上。我们的实验表明，GPC-LC接口-MALDI的组合有助于表征宽PD样品、验证低强度、高质量低聚物的存在以及检测聚合物样品中的次要序列。
