Hanton Scott D, Hyder Ingrid Z, Stets James R, Owens Kevin G, Blair William R, Guttman Charles M, Giuseppetti Anthony A
Air Products and Chemicals, Inc, Allentown, Pennsylvania 18195, USA.
J Am Soc Mass Spectrom. 2004 Feb;15(2):168-79. doi: 10.1016/j.jasms.2003.09.012.
In the interest of a more thorough understanding of the relationship between sample deposition technique and the quality of data obtained using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry, details of the electrospray (ES) process of sample deposition are investigated using a number of techniques. Sample morphology was observed with scanning electron microscopy (SEM) and atomic force microscopy (AFM), while matrix-enhanced secondary ion mass spectrometry (MESIMS) monitored surface coverage. Electrospray deposition reduces the analyte segregation that can occur during traditional dried droplet deposition for MALDI. We attribute statistically significant improvements in the reproducibility of signal intensity and MALDI average molecular mass measurements to the ES sample deposition technique.
为了更全面地理解样品沉积技术与使用基质辅助激光解吸/电离（MALDI）质谱法获得的数据质量之间的关系，我们使用多种技术研究了样品沉积的电喷雾（ES）过程细节。通过扫描电子显微镜（SEM）和原子力显微镜（AFM）观察样品形态，同时利用基质增强二次离子质谱法（MESIMS）监测表面覆盖情况。电喷雾沉积减少了在传统的MALDI干滴沉积过程中可能发生的分析物偏析。我们将信号强度和MALDI平均分子量测量的重现性在统计学上的显著提高归因于ES样品沉积技术。
