Melhus A, Tjernberg I
Department of Medical Microbiology, Lund University, Malmö University Hospital, Sweden.
APMIS. 2000 Jun;108(6):453-8. doi: 10.1034/j.1600-0463.2000.d01-82.x.
Using bacterial suspensions as simulated non-blood specimens, the capacity of three different BacT/ Alert blood culture bottles for the transportation and recovery of anaerobic bacteria with different sensitivity to air was evaluated. To better assess the performance of the BacT/Alert bottles, three other liquid media specially designed for anaerobes were included in the study. Attention was paid to recovery rates in relation to species, initial bacterial concentration, and time needed for detection. Of the BacT/Alert blood culture bottles, the anaerobic FAN bottle yielded the highest recovery rates, but its performance was limited compared with chopped meat broth in tubes. This broth allowed detection of all the tested species within 48 h. Since collection and transportation of anaerobic bacteria are of major importance for a reliable culture result, improvements are necessary.
以细菌悬液作为模拟非血液标本，评估了三种不同的BacT/Alert血培养瓶对不同空气敏感性厌氧菌的转运和复苏能力。为了更好地评估BacT/Alert瓶的性能，研究中纳入了另外三种专门为厌氧菌设计的液体培养基。重点关注了与菌种、初始细菌浓度和检测所需时间相关的复苏率。在BacT/Alert血培养瓶中，厌氧FAN瓶的复苏率最高，但与试管中的碎肉培养基相比，其性能有限。这种培养基能在48小时内检测出所有受试菌种。由于厌氧菌的采集和转运对于获得可靠的培养结果至关重要，因此有必要进行改进。
