Kataoka M, Torisu T, Tsumura H, Hirayama T, Fujikawa Y
Department of Orthopedic Surgery, Oita Medical University, Idaigaoka, Hasama-machi, Japan.
Acta Orthop Scand. 2000 Aug;71(4):414-8. doi: 10.1080/000164700317393448.
We investigated the origin of multinuclear cells (MNCs) in the granulation tissue in osteomyelitis by immunohistochemical techniques in 66 patients. 12 samples were analyzed for the presence of CD68, cathepsin K, CD11b and tartrate-resistant acid phosphatase (TRAP) activity. Many MNCs were present in the granulation tissue adjacent to a sequestrum. MNCs in contact with the sequestrum were also noted, however, no osteoblasts were found. Immunohistochemically, CD68, cathepsin K and TRAP were strongly expressed in most of the MNCs, while CD11b positive cells were not found. MNCs remote from and in contact with the sequestrum showed the same immunohistochemical features which are characteristic of osteoclasts. Further, MNCs in contact with the sequestrum had originally developed in the granulation tissue and directly infiltrated towards the sequestrum without cell-to-cell interaction with osteoblasts.
我们采用免疫组化技术对66例骨髓炎患者肉芽组织中多核细胞（MNCs）的来源进行了研究。对12份样本分析了CD68、组织蛋白酶K、CD11b和抗酒石酸酸性磷酸酶（TRAP）活性的存在情况。在死骨周围的肉芽组织中存在许多MNCs。也观察到了与死骨接触的MNCs，然而，未发现成骨细胞。免疫组化显示，大多数MNCs中CD68、组织蛋白酶K和TRAP强烈表达，而未发现CD11b阳性细胞。远离死骨和与死骨接触的MNCs显示出相同的免疫组化特征，这些特征是破骨细胞所特有的。此外，与死骨接触的MNCs最初在肉芽组织中形成，并直接向死骨浸润，未与成骨细胞发生细胞间相互作用。
