Brennan L A, Morris G M, Wasson G R, Hannigan B M, Barnett Y A
Cancer and Ageing Research Group, School of Biomedical Sciences, University of Ulster, Coleraine, Northern Ireland.
Br J Nutr. 2000 Aug;84(2):195-202. doi: 10.1017/s0007114500001422.
There is a wealth of epidemiological information on antioxidants and their possible prevention of disease progression but very little of the research on antioxidants has involved intervention studies. In this study, the potential protective effect of vitamin C or E supplementation in vivo against endogenous and H2O2-induced DNA damage levels in lymphocytes was assessed. The supplementation involved fourteen healthy male and female non-smokers mean age 25-53 (SD 1.82) years, who were asked to supplement an otherwise unchanged diet with 1000 mg vitamin C daily for 42 d or 800 mg vitamin E daily for 42 d. DNA damage in H2O2-treated peripheral blood lymphocytes (PBL) and untreated PBL before and after supplementation, and during a 6-week washout period was assessed using an ELISA. At each sampling time-point, the red cell concentrate activities of superoxide dismutase, catalase and glutathione peroxidase were also determined. Supplementation with vitamin C or vitamin E decreased significantly H2O2-induced DNA damage in PBL, but had no effect on endogenous levels of DNA damage. The activities of the antioxidant enzymes superoxide dismutase and glutathione peroxidase were suppressed during the supplementation period. These supplementation regimens may be used to limit the possible adverse effects of reactive oxygen species (including those produced during the course of an immune response) on lymphocytes in vivo, and so help to maintain their functional capacity.
关于抗氧化剂及其对疾病进展的潜在预防作用，有大量的流行病学信息，但对抗氧化剂的研究很少涉及干预性研究。在本研究中，评估了体内补充维生素C或E对淋巴细胞内源性和H2O2诱导的DNA损伤水平的潜在保护作用。参与补充的有14名健康的非吸烟男性和女性，平均年龄25 - 53（标准差1.82）岁，他们被要求在42天内每天在原本不变的饮食基础上额外补充1000毫克维生素C，或在42天内每天补充800毫克维生素E。在补充前后以及6周的洗脱期内，使用酶联免疫吸附测定法（ELISA）评估H2O2处理的外周血淋巴细胞（PBL）和未处理的PBL中的DNA损伤。在每个采样时间点，还测定了红细胞中超氧化物歧化酶、过氧化氢酶和谷胱甘肽过氧化物酶的活性。补充维生素C或维生素E可显著降低H2O2诱导的PBL中的DNA损伤，但对内源性DNA损伤水平没有影响。在补充期间，抗氧化酶超氧化物歧化酶和谷胱甘肽过氧化物酶的活性受到抑制。这些补充方案可用于限制活性氧（包括免疫反应过程中产生的活性氧）对体内淋巴细胞可能产生的不利影响，从而有助于维持其功能能力。
