Sureda Antoni, Ferrer Miguel D, Mestre Antonia, Tur Josep A, Pons Antoni
Laboratory of Physical Activity Sciences, IUNICS, University of Balearic Islands, Illes Balears, Spain.
Int J Sport Nutr Exerc Metab. 2013 Feb;23(1):31-9. doi: 10.1123/ijsnem.23.1.31.
The authors studied the effects of antioxidant diet supplementation with an almond-based beverage on neutrophil antioxidants, nitrite, and protein oxidative alterations after exercise. Fourteen trained male amateur runners were randomly assigned in a double-blind fashion to receive antioxidant supplementation (152 mg/d vitamin C and 50 mg/d vitamin E) or placebo using an almond-based beverage for 1 mo and participated in a half-marathon race. Blood samples were taken before and after the half-marathon and after 3 hr recovery. Supplementation significantly increased basal neutrophil vitamin C compared with placebo (p < .05). Exercise increased neutrophil vitamin E levels in the supplemented group and decreased vitamin C in both groups after recovery (p < .05). Neutrophil catalase and glutathione peroxidase gene expression and nitrite levels were significantly increased as result of exercise (p < .05). Nitrotyrosine and protein carbonyl derivates increased only in the placebo group after exercise (p < .05), and these values remained high at recovery. No significant differences were evidenced in caspase-3 activity and DNA damage. Antioxidant supplementation with vitamins C and E reduced the exercise-induced oxidation of proteins in neutrophils, without altering the antioxidant adaptive response, as evidenced by the increased catalase and glutathione peroxidase gene expression.
作者研究了补充以杏仁为基础的饮料的抗氧化剂饮食对运动后中性粒细胞抗氧化剂、亚硝酸盐和蛋白质氧化改变的影响。14名训练有素的男性业余跑步者以双盲方式随机分配，接受抗氧化剂补充剂（152毫克/天维生素C和50毫克/天维生素E）或使用以杏仁为基础的饮料作为安慰剂，为期1个月，并参加了一场半程马拉松比赛。在半程马拉松前后以及恢复3小时后采集血样。与安慰剂相比，补充剂显著提高了基础中性粒细胞维生素C水平（p < 0.05）。运动使补充组中性粒细胞维生素E水平升高，恢复后两组维生素C水平均降低（p < 0.05）。运动导致中性粒细胞过氧化氢酶和谷胱甘肽过氧化物酶基因表达以及亚硝酸盐水平显著升高（p < 0.05）。运动后仅安慰剂组硝基酪氨酸和蛋白质羰基衍生物增加（p < 0.05），且这些值在恢复时仍保持较高水平。半胱天冬酶-3活性和DNA损伤方面未发现显著差异。补充维生素C和E的抗氧化剂减少了运动诱导的中性粒细胞蛋白质氧化，而未改变抗氧化适应性反应，过氧化氢酶和谷胱甘肽过氧化物酶基因表达增加证明了这一点。
