Jancinová V, Drábiková K, Nosál R, Danihelová E
Institute of Experimental Pharmacology, Slovak Academy of Sciences, Bratislava, Slovak Republic.
Platelets. 2000 Aug;11(5):278-85. doi: 10.1080/09537100050129297.
Human blood platelets decreased luminol-enhanced chemiluminescence of human polymorphonuclear leukocytes (PMNL) stimulated with FMLP or Ca2+-ionophore A23187 by 56 or 47%, respectively. Horseradish peroxidase potentiated the decreasing effect of platelets on A23187-stimulated PMNL (92% inhibition) or reversed inhibition of FMLP-induced chemiluminescence to 94% potentiation, indicating dependence of platelet activity on availability of extracellular peroxidase. Moreover, platelet activity may depend also on the extent of platelet activation, as non-activated platelets (in the presence of FMLP) were found to potentiate PMNL-generated chemiluminescence, while platelets activated with A23187 displayed the opposite effect. Interference of platelets with formation and liberation of superoxide anion was indicated by platelet-modified isoluminol chemiluminescence. Superoxide dismutase with catalase and sodium azide were used, respectively, to differentiate the intracellular and the extracellular part of the chemiluminescence signal. Platelets were found to be capable of modifying both components of chemiluminescence, i.e., oxygen metabolites produced on the plasma membrane as well as on membranes of intracellular granules.
人血小板使受FMLP或钙离子载体A23187刺激的人多形核白细胞（PMNL）的鲁米诺增强化学发光分别降低了56%或47%。辣根过氧化物酶增强了血小板对A23187刺激的PMNL的降低作用（抑制率92%），或将对FMLP诱导的化学发光的抑制作用逆转至增强94%，这表明血小板活性依赖于细胞外过氧化物酶的可用性。此外，血小板活性可能还取决于血小板的活化程度，因为发现未活化的血小板（在FMLP存在下）会增强PMNL产生的化学发光，而用A23187活化的血小板则表现出相反的作用。血小板修饰异鲁米诺化学发光表明血小板对超氧阴离子的形成和释放有干扰作用。分别使用超氧化物歧化酶与过氧化氢酶和叠氮化钠来区分化学发光信号的细胞内和细胞外部分。发现血小板能够修饰化学发光的两个组成部分，即在质膜以及细胞内颗粒膜上产生的氧代谢产物。
