Stepanian R S, Aĭrapetian G S, Markarian G F, Aĭrapetian S N, Arakelian G A
Biophysics Center of Armenian National Academy of Science, Yerevan, Armenia.
Radiats Biol Radioecol. 2000 Jul-Aug;40(4):435-8.
The effect of mechanical vibrations (MV) electrical conductivity of water and optical density of aqueous DNA solution. Distilled water was treated with MV of several frequencies from 3 to 5000 Hz with an intensity of 90 dB for 30 minutes. Different sensitivities of water specific electrical conductivity (SEC) were determined (the value of distilled water SEC was 209 +/- 2 microS/m). The greatest decrease of SEC (by 15.7%) under the influence of MV was observed at the frequency of 4 Hz. There was no effect at frequencies higher than 100 Hz. The treatment of DNA water solutions with MV of frequencies 4 and 10 Hz decreased its optical density by 4.2 +/- 1.1 and 4.8 +/- 1.2% correspondingly in comparison with control. In cases of treatment with frequencies of 20 and 50 Hz no effect was observed. The mechanism of MV effect on water can be connected with the changes of system structural characteristics. It is confirmed by experiments with DNA solution, where the decrease of optical density (at 260 nm) under MV treatment is conditioned with the increase of the probability of hydrogen binding formation between the bases.
机械振动(MV)对水的电导率和DNA水溶液光密度的影响。用频率为3至5000Hz、强度为90dB的MV处理蒸馏水30分钟。测定了水的比电导率(SEC)的不同敏感性(蒸馏水SEC值为209±2微西门子/米)。在4Hz频率下,观察到MV影响下SEC的最大降幅(达15.7%)。高于100Hz的频率则无影响。用4Hz和10Hz频率的MV处理DNA水溶液,与对照相比,其光密度分别相应降低了4.2±1.1%和4.8±1.2%。在用20Hz和50Hz频率处理的情况下,未观察到影响。MV对水的作用机制可能与系统结构特征的变化有关。这在对DNA溶液的实验中得到了证实,在MV处理下,光密度(260nm处)的降低是由于碱基之间形成氢键的概率增加所致。