Vinardell M P, García L
Dept Fisiologia-Divisió IV, Facultat de Farmàcia, Barcelona, Spain.
Toxicol In Vitro. 2000 Dec;14(6):551-5. doi: 10.1016/s0887-2333(00)00050-3.
The chorioallantoic membrane-trypan blue staining assay (CAM-TBS) is used to evaluate the potential ocular irritation caused by liquid scintillation cocktails constituted by complex mixtures, including surfactants and other potential irritants. The harmful effect of these substances is determined by the amount of trypan blue adsorbed by the CAM. In the membrane previously treated with the scintillation liquids this amount was concentration dependent only in certain cases, irrespective of the water solubility of the mixtures. In general, it showed a high correlation (r=0.986) with the scores obtained in the Draize eye irritation test. In the present study, only two cocktails presented values of trypan blue adsorption higher than those recorded for their eye irritation in vivo, irrespective of the solvent nature. Unlike the classical HET-CAM procedure, this assay is objective and allows the evaluation of opaque and coloured substances without interfering in the determination of irritancy. Despite these advantages, the method is not suitable for complex mixtures of products that induce ocular irritation in small quantities.
绒毛尿囊膜-台盼蓝染色试验（CAM-TBS）用于评估由复杂混合物（包括表面活性剂和其他潜在刺激物）构成的液体闪烁鸡尾酒引起的潜在眼刺激。这些物质的有害作用由CAM吸附的台盼蓝量决定。在先前用闪烁液处理过的膜中，只有在某些情况下，该量才与浓度有关，而与混合物的水溶性无关。总体而言，它与Draize眼刺激试验中获得的分数显示出高度相关性（r=0.986）。在本研究中，无论溶剂性质如何，只有两种鸡尾酒的台盼蓝吸附值高于其体内眼刺激记录值。与经典的HET-CAM程序不同，该试验是客观的，并且允许评估不透明和有色物质，而不会干扰刺激性的测定。尽管有这些优点，但该方法不适用于少量引起眼刺激的产品的复杂混合物。
