Liao Yan, Wang Xue, Zhang Lishi, Li Guanmin
West China School of Public Health, Sichuan University, Chengdu 610041, China.
Wei Sheng Yan Jiu. 2004 May;33(3):279-83.
To establish and study the hen's egg test-chorioallantoic membrane (HET-CAM) as an alternative method of Draize eye irritation test (Draize test).
14 cosmetic ingredients were tested by the Hen's egg test-chorioallantoic membrane score (HET-CAM score) and the chorioallantoic membrane-trypan blue staining (CAM-TB) methods.
Showed that compared with two kinds of scores in Draize test, i.e. Maximum average Draize total score (MAS) and score of 24 h after application (S24), the correlation coefficient between HET-CAM score and MAS or S24 was 0.847 or 0.779, while that between CAB-TB and MAS or S24 was 0.862 or 0.831 respectively. The results also showed that CAM-TB had a higher correlation with Draize test than HET-CAM score, partly because it is objective and quantitative. Also, the results showed that HET-CAM score had the greatest correlation with conjunctivae score of the three components, and so did the CAM-TB with corneal score.
It is suggested that the two types of HET-CAM can be used in a combined manner as an effective alternative method to Draize test.
建立并研究鸡胚绒毛尿囊膜试验（HET-CAM）作为一种替代兔眼刺激试验（Draize试验）的方法。
采用鸡胚绒毛尿囊膜评分（HET-CAM评分）法和绒毛尿囊膜-台盼蓝染色（CAM-TB）法对14种化妆品成分进行检测。
结果显示，与Draize试验中的两种评分，即最大平均Draize总分（MAS）和用药后24小时评分（S24）相比，HET-CAM评分与MAS或S24的相关系数分别为0.847或0.779，而CAB-TB与MAS或S24的相关系数分别为0.862或0.831。结果还表明，CAM-TB与Draize试验的相关性高于HET-CAM评分，部分原因是它具有客观性和定量性。此外，结果表明，HET-CAM评分与三个成分中的结膜评分相关性最大，CAM-TB与角膜评分的相关性也最大。
建议将两种类型的HET-CAM联合使用，作为Draize试验的一种有效替代方法。
