[Study on using the hen's egg test-chorioallantoic membrane as an alternative method of draize eye irritation test].

OBJECTIVE

To establish and study the hen's egg test-chorioallantoic membrane (HET-CAM) as an alternative method of Draize eye irritation test (Draize test).

METHODS

14 cosmetic ingredients were tested by the Hen's egg test-chorioallantoic membrane score (HET-CAM score) and the chorioallantoic membrane-trypan blue staining (CAM-TB) methods.

RESULTS

Showed that compared with two kinds of scores in Draize test, i.e. Maximum average Draize total score (MAS) and score of 24 h after application (S24), the correlation coefficient between HET-CAM score and MAS or S24 was 0.847 or 0.779, while that between CAB-TB and MAS or S24 was 0.862 or 0.831 respectively. The results also showed that CAM-TB had a higher correlation with Draize test than HET-CAM score, partly because it is objective and quantitative. Also, the results showed that HET-CAM score had the greatest correlation with conjunctivae score of the three components, and so did the CAM-TB with corneal score.

CONCLUSION

It is suggested that the two types of HET-CAM can be used in a combined manner as an effective alternative method to Draize test.