Davidson M G, Morgan D K, McGahan M C
Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, North Carolina 27606, USA.
J Cataract Refract Surg. 2000 Oct;26(10):1550-4. doi: 10.1016/s0886-3350(99)00451-4.
To compare the effect of different cataract extraction surgical techniques on residual lens epithelial cell (LEC) density and cell regrowth rates using an in vitro model of posterior capsule opacification (PCO).
Comparative Ophthalmology Research Laboratories, North Carolina State University, Raleigh, North Carolina, USA.
Lens capsule explants were prepared from freshly enucleated canine globes after extracapsular cataract extraction (ECCE), phacoemulsification, or phacoemulsification followed by capsule vacuuming. Initial cell density on the capsule and cell proliferation were determined by phase contrast microscopy. The effects of the surgical technique on time to confluent growth of the cells across the posterior lens capsule were determined.
Residual cell density on the remaining anterior capsule immediately after lens removal was 31.6% +/- 19.3%, 16.1% +/- 8.9%, and 7.7% +/- 5.7% in the ECCE, phacoemulsification, and phacoemulsification/capsule-vacuuming groups, respectively. Time to confluence (range 5.0 to 6.3 days) was not significantly different among the 3 groups when the lens capsules were cultured in serum-supplemented media. The confluence rate was significantly longer (by approximately 5 to 7 days) in the phacoemulsification/capsule-vacuuming group than in the other 2 groups when the capsules were cultured in serum-free media.
Phacoemulsification with and without anterior and equatorial capsular vacuuming led to less initial LEC density in the capsular bag than ECCE. However, because cell proliferation rates among the 3 groups were only marginally affected, near 100% removal of LEC at the time of cataract extraction may be necessary to prevent PCO.
使用后囊膜混浊（PCO）的体外模型，比较不同白内障摘除手术技术对残留晶状体上皮细胞（LEC）密度和细胞再生率的影响。
美国北卡罗来纳州罗利市北卡罗来纳州立大学比较眼科学研究实验室。
在囊外白内障摘除术（ECCE）、超声乳化术或超声乳化术联合囊膜抽吸术后，从新鲜摘除的犬眼球制备晶状体囊膜外植体。通过相差显微镜确定囊膜上的初始细胞密度和细胞增殖情况。确定手术技术对晶状体后囊膜上细胞汇合生长时间的影响。
在ECCE、超声乳化术和超声乳化术/囊膜抽吸组中，晶状体摘除后立即在剩余前囊膜上的残留细胞密度分别为31.6%±19.3%、16.1%±8.9%和7.7%±5.7%。当晶状体囊膜在补充血清的培养基中培养时，三组之间的汇合时间（范围为5.0至6.3天）无显著差异。当囊膜在无血清培养基中培养时，超声乳化术/囊膜抽吸组的汇合率比其他两组显著延长（约5至7天）。
有或无前囊和赤道囊膜抽吸的超声乳化术导致囊袋内初始LEC密度低于ECCE。然而，由于三组之间的细胞增殖率仅受到轻微影响，白内障摘除时近乎100%清除LEC可能是预防PCO所必需的。
