Department of Clinical Sciences, North Carolina State University, Raleigh, NC 27607, USA.
J Ocul Pharmacol Ther. 2012 Aug;28(4):359-68. doi: 10.1089/jop.2011.0196. Epub 2012 Feb 28.
To determine whether celecoxib (CXB) can be released from incubated intraocular lenses (IOLs) sufficiently to inhibit lens epithelial cell (LEC) growth in an ex vivo model of posterior capsule opacification (PCO).
LEC growth was evaluated for 14 days in canine lens capsules (LCs) that had been exposed to media containing 20 μM CXB for 1-5 days. After the incubation of hydrophilic and hydrophobic IOLs in CXB solution, the determination of the in vitro release of CXB from the IOLs was performed for up to 28 days. The incubated and nonincubated IOLs were evaluated in the ex vivo model of PCO, and the rate of LEC growth was evaluated over 28 days.
The treatment of LCs with 20 μM CXB for 4 and 5 days completely inhibited LEC growth. LEC repopulation did not occur after the removal of CXB. IOLs incubated in CXB for 24 h resulted in a sustained release of CXB in vitro at levels theoretically sufficient to inhibit PCO. LCs in the ex vivo model of PCO treated with acrylic IOLs incubated in CXB had significantly suppressed LEC ingrowth compared with untreated and IOL-only LCs.
A 4-day treatment of LCs with a concentration of 20 μM CXB may effectively prevent PCO. IOLs incubated in CXB for 24 h resulted in a sustained release of CXB in vitro at levels sufficient to inhibit LEC growth in the ex vivo model of PCO. Further studies are needed to determine whether CXB-incubated IOLs can effectively prevent the development of PCO in vivo.
确定塞来昔布（CXB）能否从孵育的人工晶状体（IOL）中充分释放出来，以在后部囊膜混浊（PCO）的体外模型中抑制晶状体上皮细胞（LEC）的生长。
将犬晶状体囊（LC）暴露于含有 20μM CXB 的培养基中 1-5 天，评估 LEC 生长 14 天。在 CXB 溶液中孵育亲水和疏水 IOL 后，对 IOL 中 CXB 的体外释放进行了长达 28 天的测定。评估孵育和未孵育的 IOL 在 PCO 的体外模型中的作用，并在 28 天内评估 LEC 生长率。
用 20μM CXB 处理 LCs 4 和 5 天可完全抑制 LEC 的生长。去除 CXB 后，LEC 不会重新定植。在 CXB 中孵育 24 小时的 IOL 会导致体外持续释放 CXB，理论上足以抑制 PCO。与未处理和仅 IOL 的 LCs 相比，在 PCO 的体外模型中用孵育的 CXB 的丙烯酸 IOL 处理的 LCs 中，LEC 内生长受到明显抑制。
用浓度为 20μM CXB 处理 LCs 4 天可能有效预防 PCO。在 CXB 中孵育 24 小时的 IOL 会导致体外持续释放 CXB，其水平足以抑制 PCO 的体外模型中的 LEC 生长。需要进一步的研究来确定 CXB 孵育的 IOL 是否能有效预防体内 PCO 的发展。
