Gerashchenkov G A, Gorbunova V Iu, Zarianova L D, Rozhnova N A, Bakhitov V A
Institute of Biochemistry and Genetics, Ufa Scientific Center, Russian Academy of Sciences, Bashkortostan, Russia.
Genetika. 2000 Aug;36(8):1081-7.
Based on RAPD-PCR analysis with 15 primers including those homologous to particular loci, the level of genetic polymorphism in the collection of spring common wheat genotypes and their androclinal double haploid (ADH) lines was determined. Intraspecific polymorphism of the original wheat forms was 20%. By the absence of polymorphism in the molecular patterns of ADH lines and original forms, it was shown that, under the conditions used, no genetic changes in the genomic DNA of spring common wheat in anther culture occurred. Thus, the technology of direct in vitro androgenesis does not lead to genome rearrangements and may be used for rapid production of pure lines of such a complex allopolyploid as spring common wheat.
基于使用15种引物(包括与特定基因座同源的引物)进行的RAPD-PCR分析,测定了春性普通小麦基因型及其雄核发育双单倍体(ADH)系群体中的遗传多态性水平。原始小麦类型的种内多态性为20%。通过ADH系和原始类型的分子模式中不存在多态性,表明在所使用的条件下,花药培养中的春性普通小麦基因组DNA未发生遗传变化。因此,直接体外雄核发育技术不会导致基因组重排,可用于快速生产像春性普通小麦这样复杂的异源多倍体的纯系。