Petrova E S, Otellin V A
Institute of Experimental Medicine RAMS, St. Petersburg.
Tsitologiia. 2000;42(8):750-7.
Mechanisms of regulation of cell division in the developing neocortex are largely unknown. The aim of the present study was to investigate the influence of a microenvironment on the fetal neocortex histogenesis. The fetal neocortex from 15-day old Wistar rat embryo was grafted into the neocortex, crushed sciatic nerve and anterior chamber of eye of adult rats. A comparative study of graft development was carried out on 1, 3, 7, 10, 30 days using histological (Nissl stain, hematoxylin-eosin) and immunohistochemical (monoclonal antibody to proliferating cell nuclear antigen, and to glial fibrillary acidic protein) methods. Grafted neuroepithelial cells proliferated in grafts that developed in the neocortex and the anterior chamber of eye for 7 days, and in the sciatic nerve for 10 days. In all grafts differentiating neuroblasts, young neurons and mature neurons were observed 7, 10 and 30 days later, respectively. In 10 days, transplants in the nerve have a glial capsule, in contrast to other sites of grafting. The capsule consists of ependymocytes with microvilli and cilia 30 days later. These cells are GFAP-positive. Our results indicate epigenetic influence on the development of neuroepithelial precursors. The microenvironment of the peripheral nerve is suggested to promote glyogenesis in developing grafts. Afferent inputs do not influence the proliferative potency of brain cell precursors.
发育中的新皮质中细胞分裂的调控机制在很大程度上尚不清楚。本研究的目的是探讨微环境对胎儿新皮质组织发生的影响。将15日龄Wistar大鼠胚胎的胎儿新皮质移植到成年大鼠的新皮质、坐骨神经损伤处和眼前房中。使用组织学方法（尼氏染色、苏木精-伊红染色）和免疫组织化学方法（针对增殖细胞核抗原和胶质纤维酸性蛋白的单克隆抗体），在第1、3、7、10、30天对移植组织的发育进行了比较研究。移植的神经上皮细胞在新皮质和眼前房中发育的移植组织中增殖7天，在坐骨神经中增殖10天。在所有移植组织中，分别在7、10和30天后观察到分化的成神经细胞、年轻神经元和成熟神经元。与其他移植部位不同，10天时神经中的移植组织有一个胶质囊。30天后，该囊由具有微绒毛和纤毛的室管膜细胞组成。这些细胞GFAP呈阳性。我们的结果表明表观遗传对神经上皮前体细胞的发育有影响。提示周围神经的微环境可促进发育中移植组织的糖原生成。传入输入不影响脑细胞前体的增殖能力。
