Suh J H, Shin C S
Department of Biotechnology, College of Engineering and the Bioproducts Research Center, Yonsei University, Seoul, South Korea.
FEMS Microbiol Lett. 2000 Sep 15;190(2):241-5. doi: 10.1111/j.1574-6968.2000.tb09293.x.
During the fermentation process of Monascus 3101, coculture with Saccharomyces cerevisiae culture filtrate stimulated the formation of reproductive spores, which subsequently resulted in accelerated Monascus cell reproduction and proliferation. Protein kinase C activity was also detected. Chitinase (EC 3.2.1.14), a 120-kDa secretory protein, was purified from the S. cerevisiae culture filtrate as the effector. Monascus cells cocultured with a S. cerevisiae culture filtrate contained approximately four times more total lipids (mainly linoleic and oleic acid) than Monascus cells without coculture. Addition of exogenous fatty acids only contributed to an increase in cell mass. There was no effect on spore formation or pigment production. There were significant changes in patterns and amounts of expressed proteins in cocultured Monascus cells compared to control cells with no coculture.
在红曲霉菌3101的发酵过程中，与酿酒酵母培养滤液共培养刺激了生殖孢子的形成，这随后导致红曲霉菌细胞繁殖和增殖加速。还检测到蛋白激酶C活性。从酿酒酵母培养滤液中纯化出一种120 kDa的分泌蛋白几丁质酶（EC 3.2.1.14）作为效应物。与未共培养的红曲霉菌细胞相比，与酿酒酵母培养滤液共培养的红曲霉菌细胞总脂质（主要是亚油酸和油酸）含量大约多四倍。添加外源脂肪酸仅导致细胞量增加。对孢子形成或色素产生没有影响。与未共培养的对照细胞相比，共培养的红曲霉菌细胞中表达蛋白的模式和数量有显著变化。
