慢性丙型肝炎患者外周血单个核细胞中丙型肝炎病毒（HCV）和庚型肝炎病毒（GBV-C/HGV）RNA的检测

Detection of HCV and GBV-CHGV RNA in peripheral blood mononuclear cells of patients with chronic type C hepatitis.

作者信息

García F, García F, Roldán C, López I, Martínez N M, Alvarez M, Bernal M C, Hernández J, Maroto M C

机构信息

Department of Microbiology, Faculty of Medicine, University Hospital, Granada, Spain.

出版信息

Microbios. 2000;103(404):7-15.

PMID:11034441

Abstract

Hepatitis C virus (HCV) and hepatitis G virus (HGV) viraemia were investigated by RT-PCR protocols in peripheral blood mononuclear cells (PBMC) of 22 patients with chronic type C hepatitis. Samplings were at basal and 4-8 months after a 12 month period of treatment with interferon-alpha. A plus strand of HCV in PBMC was detected in 8 of 21 patients (38%) (p <0.05; chi2 test) with a lack of response to therapy; a minus strand was detected in 10% of chronic type C hepatitis and 25% of the patients harboured HCV RNA in PBMC. The association with a response was nearly significant (p <0.1; chi2 test). GBV-C/HGV RNA was detected in the serum of 9 of 21 (43%) patients and in PBMC of 20% of the patients viraemic. Genomic sequences of GBVC/HGV in PBMC were found, but further investigation is needed to assess the findings reported for HCV.

摘要

采用逆转录聚合酶链反应（RT-PCR）方法，对22例慢性丙型肝炎患者外周血单个核细胞（PBMC）中的丙型肝炎病毒（HCV）和庚型肝炎病毒（HGV）血症进行了研究。采样时间为基线期以及接受α干扰素治疗12个月后的4 - 8个月。在21例对治疗无反应的患者中，有8例（38%）（p<0.05；卡方检验）在PBMC中检测到HCV正链；在10%的慢性丙型肝炎患者和25%的患者的PBMC中检测到HCV负链。与治疗反应的相关性接近显著（p<0.1；卡方检验）。在21例患者中的9例（43%）血清中检测到GBV-C/HGV RNA，在病毒血症患者的PBMC中，20%检测到该病毒。在PBMC中发现了GBV-C/HGV的基因组序列，但需要进一步研究以评估所报道的HCV相关研究结果。