Yoon J H, Shin Y K, Park Y H
Int J Syst Evol Microbiol. 2000 Sep;50 Pt 5:1905-1908. doi: 10.1099/00207713-50-5-1905.
DNA-DNA relatedness of all validly described Thermoactinomyces species was determined to infer the genetic relationships between them. The levels of DNA-DNA relatedness among the type strains of Thermoactinomyces species ranged from 2-5 to 92.8%. Based on DNA relatedness data, the type strains of Thermoactinomyces intermedius, Thermoactinomyces putidus, Thermoactinomyces dichotomicus and Thermoactinomyces peptonophilus were considered to be distinct species of the genus Thermoactinomyces. However, the relationship between the type strains of Thermoactinomyces vulgaris and Thermoactinomyces candidus and the relationship between the type strains of Thermoactinomyces sacchari and Thermoactinomyces thalpophilus were reevaluated from levels of DNA-DNA relatedness. The independent DNA relatedness values between Thermoactinomyces vulgaris KCTC 9076T and Thermoactinomyces candidus KCTC 9557T were 90.8 and 92.8%. Thermoactinomyces thalpophilus KCTC 9789T and Thermoactinomyces sacchari KCTC 9790T exhibited independent values of 85.6 and 87.3%. Accordingly, on the basis of DNA-DNA relatedness data together with 16S rDNA sequence data determined recently, it is proposed that Thermoactinomyces candidus should be considered as a synonym of Thermoactinomyces vulgaris and Thermoactinomyces thalpophilus be considered as a synonym of Thermoactinomyces sacchari.
测定了所有有效描述的嗜热放线菌物种之间的DNA-DNA相关性，以推断它们之间的遗传关系。嗜热放线菌物种模式菌株之间的DNA-DNA相关性水平在2.5%至92.8%之间。基于DNA相关性数据，中间嗜热放线菌、恶臭嗜热放线菌、双歧嗜热放线菌和嗜胨嗜热放线菌的模式菌株被认为是嗜热放线菌属的不同物种。然而，从DNA-DNA相关性水平重新评估了普通嗜热放线菌和白色嗜热放线菌模式菌株之间的关系以及甘蔗嗜热放线菌和嗜盐嗜热放线菌模式菌株之间的关系。普通嗜热放线菌KCTC 9076T和白色嗜热放线菌KCTC 9557T之间的独立DNA相关性值分别为90.8%和92.8%。嗜盐嗜热放线菌KCTC 9789T和甘蔗嗜热放线菌KCTC 9790T的独立值分别为85.6%和87.3%。因此，基于DNA-DNA相关性数据以及最近测定的16S rDNA序列数据，建议将白色嗜热放线菌视为普通嗜热放线菌的同义词，将嗜盐嗜热放线菌视为甘蔗嗜热放线菌的同义词。
