霍乱弧菌疫苗株中的蛋白酶活性与极化的T84肠上皮细胞跨细胞上皮电阻降低之间的关联。
Association of protease activity in Vibrio cholerae vaccine strains with decreases in transcellular epithelial resistance of polarized T84 intestinal epithelial cells.
作者信息
Mel S F, Fullner K J, Wimer-Mackin S, Lencer W I, Mekalanos J J
机构信息
Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115, USA.
出版信息
Infect Immun. 2000 Nov;68(11):6487-92. doi: 10.1128/IAI.68.11.6487-6492.2000.
Abstract
Culture supernatants prepared from reactogenic strains of Vibrio cholerae cause a decrease in the transcellular epithelial resistance of T84 intestinal cells. This decrease correlates with the presence of hemagglutinin/protease but not with the presence of other potential accessory toxins or proteases. These data suggest a possible role for hemagglutinin/protease in reactogenicity, although other factors may also contribute.
摘要
从霍乱弧菌反应原性菌株制备的培养上清液会导致T84肠细胞的跨细胞上皮电阻降低。这种降低与血凝素/蛋白酶的存在相关，而与其他潜在辅助毒素或蛋白酶的存在无关。这些数据表明血凝素/蛋白酶在反应原性中可能起作用，尽管其他因素也可能有贡献。
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