Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig, 44519, Egypt.
BMC Microbiol. 2022 Apr 21;22(1):108. doi: 10.1186/s12866-022-02509-x.
Biopriming as a new technique of seed treatment involves the application of beneficial microorganisms on the seed surface to stimulate seed germination, plant growth, and protect the seed from soil and seed-borne pathogens. The present investigation was carried out on seed germination, seedling vigor and biochemical traits of one of the most important vegetable crops (Tomato, Solanum lycopersicum L.). The treatments comprised viz. T1: Non primed seeds (Control), T2: Hydropriming, T3: Biopriming with C-phycocyanin (C-PC) (Spirulina platensis extract), T4: Biopriming with Trichoderma asperellum, T5: Biopriming with T. viride, T6: Biopriming with Beauveria bassiana.
Extraction and purification of C-phycocyanin (C-PC) from the dry S. platensis powder using various methods was performed. The purity after dialyses was 0.49 and its ultimate purity (A/A) after ion-exchange chromatography was 4.64. The results on tomato seedlings revealed that the maximum germination percentage (100%), germination index (15.46 and 15.12), seedling length (10.67 cm), seedling dry weight (1.73 and 1.97 mg) and seedling length vigor index (1066.7) were recorded for tomato biopriming with T. viride, and B. bassiana (T5 and T6). Moreover, the quantitative estimation of total carbohydrates and total free amino acids contents in bioprimed tomato seedlings indicated a significantly higher amount with T. viride, followed by those bioprimed with T. asperellum, B. bassiana and C-PC extract.
Thus, our results indicated that biopriming of tomato seeds with beneficial fungal inoculants and C-PC was very effective. The most operative biostimulants were those bioprimed with T. viride and B. bassiana compared to other biostimulants (T. asperellum and C-PC). Therefore, to ensure sustainable agriculture, this study offers new possibilities for the biopriming application as an alternative and ecological management strategy to chemical treatment and provides a valuable basis for improving seed germination.
生物引发作为一种新的种子处理技术，涉及将有益微生物应用于种子表面，以刺激种子发芽、植物生长，并保护种子免受土壤和种子传播病原体的侵害。本研究对一种重要的蔬菜作物（番茄，Solanum lycopersicum L.）的种子发芽、幼苗活力和生化特性进行了研究。处理包括：T1：未引发的种子（对照）、T2：水引发、T3：用 C-藻蓝蛋白（C-PC）（螺旋藻提取物）进行生物引发、T4：用木霉属 Asperellum 进行生物引发、T5：用木霉菌进行生物引发、T6：用白僵菌进行生物引发。
使用各种方法从干螺旋藻粉中提取和纯化 C-藻蓝蛋白（C-PC）。透析后的纯度为 0.49，离子交换层析后的最终纯度（A/A）为 4.64。番茄幼苗的结果表明，番茄用木霉菌和白僵菌（T5 和 T6）进行生物引发时，最大发芽率（100%）、发芽指数（15.46 和 15.12）、幼苗长度（10.67 cm）、幼苗干重（1.73 和 1.97 mg）和幼苗长度活力指数（1066.7）最高。此外，生物引发的番茄幼苗中总碳水化合物和总游离氨基酸含量的定量估计表明，木霉菌引发的含量显著更高，其次是木霉菌属、白僵菌和 C-PC 提取物引发的含量。
因此，我们的结果表明，用有益真菌接种剂和 C-PC 对番茄种子进行生物引发非常有效。与其他生物刺激剂（木霉菌属和 C-PC）相比，用木霉菌和白僵菌生物引发的效果最为显著。因此，为了确保可持续农业，本研究为生物引发作为一种替代和生态管理策略的应用提供了新的可能性，为提高种子发芽率提供了有价值的基础。
