• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

相似文献

1
Resistance to methotrexate due to AcrAB-dependent export from Escherichia coli.由于大肠杆菌中AcrAB依赖性外排导致对甲氨蝶呤产生耐药性。
Antimicrob Agents Chemother. 2000 Nov;44(11):3210-2. doi: 10.1128/AAC.44.11.3210-3212.2000.
2
Evidence that TolC is required for functioning of the Mar/AcrAB efflux pump of Escherichia coli.有证据表明，TolC是大肠杆菌Mar/AcrAB外排泵发挥功能所必需的。
J Bacteriol. 1996 Oct;178(19):5803-5. doi: 10.1128/jb.178.19.5803-5805.1996.
3
AcrB-AcrA Fusion Proteins That Act as Multidrug Efflux Transporters.作为多药外排转运蛋白的AcrB-AcrA融合蛋白。
J Bacteriol. 2015 Nov 2;198(2):332-42. doi: 10.1128/JB.00587-15. Print 2016 Jan 15.
4
The C-terminal domain of AcrA is essential for the assembly and function of the multidrug efflux pump AcrAB-TolC.AcrA的C末端结构域对于多药外排泵AcrAB-TolC的组装和功能至关重要。
J Bacteriol. 2009 Jul;191(13):4365-71. doi: 10.1128/JB.00204-09. Epub 2009 May 1.
5
Genetic evidence for functional interactions between TolC and AcrA proteins of a major antibiotic efflux pump of Escherichia coli.大肠杆菌主要抗生素外排泵的TolC和AcrA蛋白之间功能相互作用的遗传证据。
Mol Microbiol. 2004 Nov;54(3):620-31. doi: 10.1111/j.1365-2958.2004.04301.x.
6
Entry into and release of solvents by Escherichia coli in an organic-aqueous two-liquid-phase system and substrate specificity of the AcrAB-TolC solvent-extruding pump.大肠杆菌在有机-水双液相系统中对溶剂的摄入与释放以及AcrAB-TolC溶剂外排泵的底物特异性
J Bacteriol. 2000 Sep;182(17):4803-10. doi: 10.1128/JB.182.17.4803-4810.2000.
7
Interactions underlying assembly of the Escherichia coli AcrAB-TolC multidrug efflux system.大肠杆菌AcrAB-TolC多药外排系统组装的潜在相互作用。
Mol Microbiol. 2004 Jul;53(2):697-706. doi: 10.1111/j.1365-2958.2004.04158.x.
8
Toxic waste disposal in Escherichia coli.大肠杆菌中的有毒废物处理
J Bacteriol. 2002 Jul;184(13):3699-703. doi: 10.1128/JB.184.13.3699-3703.2002.
9
Interaction between the TolC and AcrA proteins of a multidrug efflux system of Escherichia coli.大肠杆菌多药外排系统中TolC与AcrA蛋白之间的相互作用。
J Bacteriol. 2004 Dec;186(24):8533-6. doi: 10.1128/JB.186.24.8533-8536.2004.
10
Functional relationships between the AcrA hairpin tip region and the TolC aperture tip region for the formation of the bacterial tripartite efflux pump AcrAB-TolC.AcrA 发夹尖端区域与 TolC 孔口尖端区域之间的功能关系，用于形成细菌三组分外排泵 AcrAB-TolC。
J Bacteriol. 2010 Sep;192(17):4498-503. doi: 10.1128/JB.00334-10. Epub 2010 Jun 25.

引用本文的文献

1
3‑Hydroxy-Beta-Lactam Inhibitors of Dihydrofolate Synthetase.二氢叶酸合成酶的3-羟基-β-内酰胺抑制剂。
ACS Bio Med Chem Au. 2025 Jun 5;5(4):637-649. doi: 10.1021/acsbiomedchemau.5c00036. eCollection 2025 Aug 20.
2
Studying Target-Engagement of Anti-Infectives by Solvent-Induced Protein Precipitation and Quantitative Mass Spectrometry.通过溶剂诱导蛋白沉淀和定量质谱法研究抗感染药物的靶点结合情况
ACS Infect Dis. 2024 Dec 13;10(12):4087-4102. doi: 10.1021/acsinfecdis.4c00417. Epub 2024 Nov 20.
3
The impact of the human gut microbiome on the treatment of autoimmune disease.人类肠道微生物组对自身免疫性疾病治疗的影响。
Immunol Rev. 2024 Aug;325(1):107-130. doi: 10.1111/imr.13358. Epub 2024 Jun 12.
4
Interplay between inflammatory bowel disease therapeutics and the gut microbiome reveals opportunities for novel treatment approaches.炎症性肠病治疗方法与肠道微生物群之间的相互作用揭示了新型治疗方法的机会。
Microbiome Res Rep. 2023 Sep 26;2(4):35. doi: 10.20517/mrr.2023.41.
5
Advances in the implications of the gut microbiota on the treatment efficacy of disease-modifying anti-rheumatic drugs in rheumatoid arthritis.肠道微生物群对类风湿关节炎疾病修饰抗风湿药物治疗效果影响的研究进展。
Front Immunol. 2023 Sep 28;14:1189036. doi: 10.3389/fimmu.2023.1189036. eCollection 2023.
6
SIMMER employs similarity algorithms to accurately identify human gut microbiome species and enzymes capable of known chemical transformations.SIMMER 采用相似性算法来准确识别人类肠道微生物物种和能够进行已知化学转化的酶。
Elife. 2023 Jun 12;12:e82401. doi: 10.7554/eLife.82401.
7
Current evidence and clinical relevance of drug-microbiota interactions in inflammatory bowel disease.炎症性肠病中药物与微生物群相互作用的当前证据及临床相关性。
Front Microbiol. 2023 Feb 23;14:1107976. doi: 10.3389/fmicb.2023.1107976. eCollection 2023.
8
Massively parallel, computationally guided design of a proenzyme.大规模并行，计算指导的酶原设计。
Proc Natl Acad Sci U S A. 2022 Apr 12;119(15):e2116097119. doi: 10.1073/pnas.2116097119. Epub 2022 Apr 4.
9
The chemotherapeutic drug methotrexate selects for antibiotic resistance.化疗药物甲氨蝶呤选择出抗生素耐药性。
EBioMedicine. 2021 Dec;74:103742. doi: 10.1016/j.ebiom.2021.103742. Epub 2021 Dec 11.
10
Methotrexate impacts conserved pathways in diverse human gut bacteria leading to decreased host immune activation.甲氨蝶呤影响多种人类肠道细菌中保守的途径，导致宿主免疫激活减少。
Cell Host Microbe. 2021 Mar 10;29(3):362-377.e11. doi: 10.1016/j.chom.2020.12.008. Epub 2021 Jan 12.

本文引用的文献

1
Involvement of outer membrane protein TolC, a possible member of the mar-sox regulon, in maintenance and improvement of organic solvent tolerance of Escherichia coli K-12.外膜蛋白TolC（mar-sox调控子的一个可能成员）参与维持和提高大肠杆菌K-12对有机溶剂的耐受性。
J Bacteriol. 1998 Feb;180(4):938-44. doi: 10.1128/JB.180.4.938-944.1998.
2
The complete genome sequence of Escherichia coli K-12.大肠杆菌K-12的全基因组序列。
Science. 1997 Sep 5;277(5331):1453-62. doi: 10.1126/science.277.5331.1453.
3
Molecular typing of enterohemorrhagic Escherichia coli O157:H7 isolates in Japan by using pulsed-field gel electrophoresis.运用脉冲场凝胶电泳对日本肠出血性大肠杆菌O157:H7分离株进行分子分型。
J Clin Microbiol. 1997 Jul;35(7):1675-80. doi: 10.1128/jcm.35.7.1675-1680.1997.
4
Multidrug efflux pumps of gram-negative bacteria.革兰氏阴性菌的多药外排泵
J Bacteriol. 1996 Oct;178(20):5853-9. doi: 10.1128/jb.178.20.5853-5859.1996.
5
Evidence that TolC is required for functioning of the Mar/AcrAB efflux pump of Escherichia coli.有证据表明，TolC是大肠杆菌Mar/AcrAB外排泵发挥功能所必需的。
J Bacteriol. 1996 Oct;178(19):5803-5. doi: 10.1128/jb.178.19.5803-5805.1996.
6
Molecular cloning and characterization of acrA and acrE genes of Escherichia coli.大肠杆菌acrA和acrE基因的分子克隆与特性分析
J Bacteriol. 1993 Oct;175(19):6299-313. doi: 10.1128/jb.175.19.6299-6313.1993.
7
XbaI and BlnI genomic cleavage maps of Escherichia coli K-12 strain MG1655 and comparative analysis of other strains.大肠杆菌K-12菌株MG1655的XbaI和BlnI基因组切割图谱及其他菌株的比较分析
J Mol Biol. 1993 Jul 20;232(2):419-45. doi: 10.1006/jmbi.1993.1401.
8
Antibiotic-supersusceptible mutants of Escherichia coli and Salmonella typhimurium.大肠杆菌和鼠伤寒沙门氏菌的抗生素超敏感突变体。
Antimicrob Agents Chemother. 1993 Nov;37(11):2255-60. doi: 10.1128/AAC.37.11.2255.
9
Multidrug resistance pumps in bacteria: variations on a theme.细菌中的多药耐药泵：同一主题的变体
Trends Biochem Sci. 1994 Mar;19(3):119-23. doi: 10.1016/0968-0004(94)90204-6.
10
Penicillin-binding proteins are regulated by rpoS during transitions in growth states of Escherichia coli.在大肠杆菌生长状态转变过程中，青霉素结合蛋白受rpoS调控。
Antimicrob Agents Chemother. 1994 Feb;38(2):205-10. doi: 10.1128/AAC.38.2.205.

由于大肠杆菌中AcrAB依赖性外排导致对甲氨蝶呤产生耐药性。

Resistance to methotrexate due to AcrAB-dependent export from Escherichia coli.

作者信息

Kopytek S J, Dyer J C, Knapp G S, Hu J C

机构信息

Department of Biochemistry and Biophysics and Center for Advanced Biomolecular Research, Texas A&M University, College Station, Texas 77843-2128, USA.

出版信息

Antimicrob Agents Chemother. 2000 Nov;44(11):3210-2. doi: 10.1128/AAC.44.11.3210-3212.2000.

DOI:10.1128/AAC.44.11.3210-3212.2000
PMID:11036056
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC101636/
Abstract

Many laboratory strains of Escherichia coli are resistant to methotrexate (MTX), a folate analogue that binds dihydrofolate reductase (DHFR). Mutations that inactivate either tolC or acrA confer MTX sensitivity. Further, overexpression of a fusion protein with DHFR activity reverses this sensitivity by titrating out intracellular MTX. These results suggest that MTX accumulates in cells where mutations in acrA or tolC have inactivated the TolC-dependent AcrAB multidrug resistance efflux pump.

摘要

许多大肠杆菌实验室菌株对甲氨蝶呤（MTX）具有抗性，甲氨蝶呤是一种结合二氢叶酸还原酶（DHFR）的叶酸类似物。使tolC或acrA失活的突变会导致对MTX敏感。此外，具有DHFR活性的融合蛋白的过表达通过滴定细胞内MTX来逆转这种敏感性。这些结果表明，MTX在acrA或tolC发生突变使依赖于TolC的AcrAB多药耐药性外排泵失活的细胞中积累。