Darbin O, Lonjon M, Quentien M H, Michiels J F, Grellier P, Negrin J, Rostain J C, Risso J J
Université de la Méditerranée, Laboratoire de barobiologie et de neurochimie des interactions cellulaires, Institut J. Roche, Faculté de médecine Nord. 13916 Cedex 20, Marseille, France.
Brain Res. 2000 Oct 27;881(2):121-7. doi: 10.1016/s0006-8993(00)02633-0.
The purpose of this study is to investigate the in vivo tumoral brain metabolism in free moving rats using microdialysis. Cells from C6 glioma cell line were inoculated in one striatum 15 days before the microdialysis experimentation. Then, using a new system allowing perfusion of several microdialysis probes in free moving rat, normalised dialysate levels of glucose, lactate and pyruvate were monitored in both glioma and control striatum. At the end of the procedure, animals were sacrificed for histological study. Data shows that probe functioning is similar in both tissues. The results for normalised glucose level were in striatum control: 2.14 mM, in tumoral striatum: 1.71 mM (P>0.1); for lactate, respectively, 0.86 and 1.65 mM (P<0.05) and for pyruvate, respectively, 65.56 and 140. 94 microM (P<0.05). This data clearly shows a significant increase of pyruvate and lactate in tumoral striatum compared to normal striatum, correlating previous in vitro studies on glioma metabolism. We conclude that this microdialysis technique is of value in tumoral brain and could constitute an interesting tool for a better understanding of glioma metabolism.
本研究的目的是使用微透析技术研究自由活动大鼠体内的肿瘤脑代谢。在微透析实验前15天，将C6胶质瘤细胞系的细胞接种于一侧纹状体。然后，使用一种新系统，该系统允许在自由活动的大鼠体内灌注多个微透析探针，监测胶质瘤和对照纹状体中葡萄糖、乳酸和丙酮酸的透析液标准化水平。实验结束时，处死动物进行组织学研究。数据显示两个组织中的探针功能相似。标准化葡萄糖水平的结果为：对照纹状体中为2.14 mM，肿瘤纹状体中为1.71 mM（P>0.1）；乳酸水平分别为0.86和1.65 mM（P<0.05），丙酮酸水平分别为65.56和140.94 microM（P<0.05）。该数据清楚地表明，与正常纹状体相比，肿瘤纹状体中的丙酮酸和乳酸显著增加，这与先前关于胶质瘤代谢的体外研究结果相关。我们得出结论，这种微透析技术在肿瘤脑研究中具有价值，可能成为更好地理解胶质瘤代谢的一个有趣工具。
