α-CaMKII的3'非翻译区中的两个顺式作用元件调节其树突靶向。

Two cis-acting elements in the 3' untranslated region of alpha-CaMKII regulate its dendritic targeting.

作者信息

Mori Y, Imaizumi K, Katayama T, Yoneda T, Tohyama M

机构信息

Department of Anatomy and Neuroscience, Graduate School of Medicine, Osaka University, 2-2 Yamadaoka Suita, Osaka 565-0871, Japan.

出版信息

Nat Neurosci. 2000 Nov;3(11):1079-84. doi: 10.1038/80591.

DOI:10.1038/80591

PMID:11036263

Abstract

Dendritic localization of the alpha subunit of Ca2+/calmodulin-dependent protein kinase II (alphaCaMKII) mRNA in CNS neurons requires its 3' untranslated region (3'UTR). We investigated this targeting mechanism by identifying two cis-acting elements in the 3'UTR. One is a 30-nucleotide element that mediated dendritic translocation. A homologous sequence in the 3'UTR of neurogranin, transcripts of which also reside in dendrites, also funtioned in cis to promote its dendritic transport. Other putative elements in the alphaCaMKII mRNA inhibit its transport in a resting state. This inhibition was removed in depolarized neurons, and such activity-dependent derepression was a primary requirement for their dendritic targeting.

摘要

Ca2+/钙调蛋白依赖性蛋白激酶II（αCaMKII）mRNA的α亚基在中枢神经系统神经元中的树突定位需要其3'非翻译区（3'UTR）。我们通过鉴定3'UTR中的两个顺式作用元件来研究这种靶向机制。一个是介导树突转运的30个核苷酸元件。神经颗粒素3'UTR中的同源序列，其转录本也存在于树突中，也以顺式作用促进其树突运输。αCaMKII mRNA中的其他假定元件在静息状态下抑制其运输。这种抑制在去极化神经元中被消除，并且这种活性依赖性去抑制是它们树突靶向的主要要求。