Ross E L, D'Cruz D, Morrow W J
Department of Immunology, Royal Hospitals NHS Trust, London, UK.
J Rheumatol. 2000 Oct;27(10):2432-43.
To examine normal and psoriatic skin and synovial tissue from patients with psoriatic arthritis (PsA) for evidence of monocyte chemotactic protein-1 (MCP-1) mediated T cell chemotaxis.
Peripheral blood (PB), synovial fluid (SF), normal and psoriatic skin, and synovial biopsies were obtained from patients with PsA (n = 19) and compared to samples from normal (n = 5) and disease (n = 5) controls (NC, DC). Immune cell populations in PB and SF samples were assessed by immunofluorescent labeling and flow cytometry, levels of soluble MCP-1 were determined by quantitative ELISA, and immunohistochemistry was used to detect T cell subsets and macrophages and MCP-1 protein in frozen skin and synovial tissue sections.
CD8+ but not CD4+ T cells were elevated in SF compared to PB, and the majority of these cells expressed CD45RO. Plasma MCP-1 levels in PsA were elevated relative to NC. MCP-1 levels were significantly higher than paired plasma samples in patients with recent onset (< 6 mo) synovitis (n = 10). A positive correlation was observed between synovial T cell numbers and MCP-1 levels in SF. MCP-1 protein was present in all tissues examined, but most intense expression was observed in synovium.
Elevated concentrations of MCP-1 concomitant with memory T cell infiltration in PsA SF suggests that MCP-1 mediated chemotaxis is involved in the recruitment of T lymphocytes into the synovial compartment of patients with PsA.
检测银屑病关节炎（PsA）患者的正常皮肤和银屑病皮肤以及滑膜组织，寻找单核细胞趋化蛋白-1（MCP-1）介导的T细胞趋化性证据。
从19例PsA患者获取外周血（PB）、滑液（SF）、正常皮肤和银屑病皮肤以及滑膜活检组织，并与5例正常对照（NC）和5例疾病对照（DC）的样本进行比较。通过免疫荧光标记和流式细胞术评估PB和SF样本中的免疫细胞群体，用定量ELISA法测定可溶性MCP-1水平，并用免疫组织化学法检测冰冻皮肤和滑膜组织切片中的T细胞亚群、巨噬细胞和MCP-1蛋白。
与PB相比，SF中的CD8⁺而非CD4⁺T细胞升高，且这些细胞大多数表达CD45RO。PsA患者的血浆MCP-1水平相对于NC升高。近期发病（<6个月）滑膜炎患者（n = 10）的MCP-1水平显著高于配对的血浆样本。SF中的滑膜T细胞数量与MCP-1水平之间存在正相关。所有检测组织中均存在MCP-1蛋白，但滑膜中的表达最为强烈。
PsA患者SF中MCP-1浓度升高并伴有记忆性T细胞浸润，提示MCP-1介导的趋化作用参与了T淋巴细胞向PsA患者滑膜腔的募集。
