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免疫荧光和免疫过氧化物酶技术中直接法与间接法的敏感性:一项对比研究(作者译)

[The sensitivity of direct and indirect methods in immunofluorescence and immunoperoxidase techniques: a comparative study (author's transl)].

作者信息

Bellon B, Sapin C, Druet P

出版信息

Ann Immunol (Paris). 1975 Jan;126(1):15-22.

PMID:1103716
Abstract

A comparative study of the sensitivities of the immunofluorescence and immunoperoxidase techniques was undertaken. The material used was tissue from experimental and human glomerulonephritis. With the direct method, immunoperoxidase was 10 to 30 times more sensitive than immunofluorescence. However, the sensitivities became equivalent if the level of the labeling in immunofluorescence was equal to or superior to 6. In the indirect method, the sensitivity of the immunoperoxidase technique was one to ten-fold greater than with immunofluorescence.

摘要

对免疫荧光技术和免疫过氧化物酶技术的敏感性进行了一项比较研究。所用材料为来自实验性和人类肾小球肾炎的组织。采用直接法时,免疫过氧化物酶的敏感性比免疫荧光高10至30倍。然而,如果免疫荧光中的标记水平等于或高于6,则敏感性变得相当。采用间接法时,免疫过氧化物酶技术的敏感性比免疫荧光高1至10倍。

相似文献

1
[The sensitivity of direct and indirect methods in immunofluorescence and immunoperoxidase techniques: a comparative study (author's transl)].免疫荧光和免疫过氧化物酶技术中直接法与间接法的敏感性:一项对比研究(作者译)
Ann Immunol (Paris). 1975 Jan;126(1):15-22.
2
[Immunofluorescence and immunoperoxidase: a comparison for sensitivity in cutaneous immunopathology (author's transl)].免疫荧光法与免疫过氧化物酶法:皮肤免疫病理学中敏感性的比较(作者译)
Ann Dermatol Venereol. 1978 Oct;105(10):793-8.
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[Glomerular immunopathology: comparative study with immunofluorescence and immunoperoxidase (author's transl)].[肾小球免疫病理学:免疫荧光与免疫过氧化物酶的比较研究(作者译)]
Rev Bras Pesqui Med Biol. 1979 Dec;12(6):377-81.
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[Prozone phenomenon in immunofluorescence and immunoperoxidase: a possible mechanism (author's transl)].
Ann Immunol (Paris). 1974 Nov;125(6):871-84.
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[Assay of the activity of horseradish peroxidase antibodies using ammonium sulfate technique (author's transl)].[采用硫酸铵技术检测辣根过氧化物酶抗体的活性(作者译)]
Ann Immunol (Paris). 1976 Jan-Feb;127(1):3-10.
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Detection of immunoglobulin depositions by immunofluorescence and/or immunoperoxidase (P-AP method) in patients with IgA nephropathy.通过免疫荧光和/或免疫过氧化物酶(P-AP法)检测IgA肾病患者的免疫球蛋白沉积。
Tokai J Exp Clin Med. 1987 Dec;12(5-6):343-7.
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[The value of immunofluorescence on frozen sections and immunoperoxidase methods on paraffin sections with different types of glomerulonephritis comparative study].
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[A method for the photometric determination of the immunofluorescence intensity of the glomerulonephritis (author's transl)].
Z Immunitatsforsch Exp Klin Immunol. 1972 Dec;144(4):323-8.
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Ann Sclavo. 1979 Jul-Aug;21(4):561-7.
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[Immuno-electron microscopic study of membranous glomerulonephritis by immunoperoxidase method (author's transl)].用免疫过氧化物酶法对膜性肾小球肾炎进行免疫电子显微镜研究(作者译)
Nihon Jinzo Gakkai Shi. 1978 Sep;20(9):943-53.