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免疫荧光和免疫过氧化物酶技术中直接法与间接法的敏感性:一项对比研究(作者译)

[The sensitivity of direct and indirect methods in immunofluorescence and immunoperoxidase techniques: a comparative study (author's transl)].

作者信息

Bellon B, Sapin C, Druet P

出版信息

Ann Immunol (Paris). 1975 Jan;126(1):15-22.

PMID:1103716
Abstract

A comparative study of the sensitivities of the immunofluorescence and immunoperoxidase techniques was undertaken. The material used was tissue from experimental and human glomerulonephritis. With the direct method, immunoperoxidase was 10 to 30 times more sensitive than immunofluorescence. However, the sensitivities became equivalent if the level of the labeling in immunofluorescence was equal to or superior to 6. In the indirect method, the sensitivity of the immunoperoxidase technique was one to ten-fold greater than with immunofluorescence.

摘要

对免疫荧光技术和免疫过氧化物酶技术的敏感性进行了一项比较研究。所用材料为来自实验性和人类肾小球肾炎的组织。采用直接法时,免疫过氧化物酶的敏感性比免疫荧光高10至30倍。然而,如果免疫荧光中的标记水平等于或高于6,则敏感性变得相当。采用间接法时,免疫过氧化物酶技术的敏感性比免疫荧光高1至10倍。

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