Gisler R H, Pagès J M, Bussard A E
Ann Immunol (Paris). 1975 Feb-Mar;126(2):231-8.
If cultured over 10 days, peritoneal cells from different strains-CBA/J, C3H, C57B1, DBA/2, Balb/C, NZB and congenitally athymic Nude (Nu+/Nu+), fifth backcross generation with Balb/C-of unimmunized mice developed high numbers of plaque-forming cells against sheep erythrocytes (up to 13 per cent of the recovered viable cells at day 6) without being triggered by antigen. Plaque-forming cells could be demonstrated by all methods of local hemolysis (agarose, liquid medium, CMC). This capacity increase with the age of the donor mice. The phenomenon is immunological, as plaque formation could be inhibited by specific anti-mouse IgM serum and is complement-dependent. It also displays immunological specificity: the erythrocytes from sheep, goat, cow, which cross react when tested in the classical mouse immune spleen cells system, give hemolysis plaques with cultured mouse peritoneal cells, while horse, rabbit rat erythrocytes, non cross reacting with sheep erythrocytes in the classical immune systems, give no plaques with peritoneal cells. The removal of cells adherent to glass does not diminish the ability of the peritoneal cell population to form plaques against sheep erythrocytes. These results suggest that peritoneal lymphocytes constitute a highly specialized population of already programmed cells which become derepressed when removed from their natural environment and exposed to cell-culture conditions.
如果培养超过10天，来自不同品系未免疫小鼠（CBA/J、C3H、C57B1、DBA/2、Balb/C、NZB以及与Balb/C回交五代的先天性无胸腺裸鼠（Nu+/Nu+））的腹膜细胞会产生大量针对绵羊红细胞的噬斑形成细胞（在第6天高达回收活细胞的13%），且无需抗原触发。所有局部溶血方法（琼脂糖、液体培养基、羧甲基纤维素）都能检测到噬斑形成细胞。这种能力随着供体小鼠年龄的增长而增强。该现象具有免疫性，因为噬斑形成可被特异性抗小鼠IgM血清抑制且依赖补体。它还表现出免疫特异性：在经典小鼠免疫脾细胞系统中检测时发生交叉反应的绵羊、山羊、牛的红细胞，能与培养的小鼠腹膜细胞产生溶血噬斑，而在经典免疫系统中与绵羊红细胞不发生交叉反应的马、兔、大鼠红细胞，与腹膜细胞则不产生噬斑。去除贴壁于玻璃的细胞并不会降低腹膜细胞群体针对绵羊红细胞形成噬斑的能力。这些结果表明，腹膜淋巴细胞构成了一个高度特化的已编程细胞群体，当它们从自然环境中移出并暴露于细胞培养条件时会去抑制。
