Dauphinée M, Talal N
J Clin Lab Immunol. 1979 Feb;1(4):355-60.
New Zealand Black mice spontaneously develop autoimmune hemolytic anemia. Peritoneal cavity lymphocytes from NZB mice include a high proportion of cells that bind autologous erythrocytes. These cells produce ani-erythrocyte antibody that can be demonstrated when target erythrocytes are treated with bromelin. Such autoreactive cells are also present in normal mice, though in much smaller numbers. Aging and sex influence the peritoneal anti-erythrocyte response to bromelin-treated mouse erythrocytes. Older mice generally produce more rosettes and plaques than younger animals, and females have higher responses than males. During short-term culture in the absence of antigen, the response to bromelin-treated mouse erythrocytes increases. By contrast, the response to untreated mouse erythrocytes decreases during the same culture conditions. The in vitro response to bromelin-treated mouse erythrocytes by New Zealand Black peritoneal lymphocytes is suppressed by a cell that binds to untreated erythrocytes. The enhanced response to bromelin-treated mouse erythrocytes in culture may reflect the loss of this suppressor cell.
新西兰黑鼠会自发患上自身免疫性溶血性贫血。来自新西兰黑鼠的腹腔淋巴细胞中，有很大比例的细胞能与自体红细胞结合。这些细胞产生抗红细胞抗体，当靶红细胞用菠萝蛋白酶处理时就能显示出来。这种自身反应性细胞在正常小鼠中也存在，不过数量要少得多。衰老和性别会影响腹腔对经菠萝蛋白酶处理的小鼠红细胞的抗红细胞反应。老年小鼠通常比幼年动物产生更多的玫瑰花结和噬斑，并且雌性的反应比雄性更高。在无抗原的短期培养过程中，对经菠萝蛋白酶处理的小鼠红细胞的反应会增强。相比之下，在相同培养条件下，对未经处理的小鼠红细胞的反应会减弱。新西兰黑鼠腹腔淋巴细胞对经菠萝蛋白酶处理的小鼠红细胞的体外反应会被一种能与未经处理的红细胞结合的细胞所抑制。培养中对经菠萝蛋白酶处理的小鼠红细胞反应增强可能反映了这种抑制性细胞的缺失。
