Newman J S, O'Brien R T
Appl Microbiol. 1975 Oct;30(4):584-8. doi: 10.1128/am.30.4.584-588.1975.
A gas chromatographic procedure which shows promise as a presumptive test for coliform bacteria in water is described. Total coliform bacteria concentrations were determined from the incubation times at 37 C required for ethanol to be produced. Fecal coliform densities were determined in a similar manner at 44.5 C. The culture medium was filter sterilized M-9 salts supplemented with 1% lactose, 0.1% Casamino Acids, and 0.1% yeast extract. Best results were obtained when the initial total coliform concentrations were 5 per ml or higher and when fecal coliform concentrations were 50 per ml or higher. Minimum detection times at these concentrations were 9 and 12 h, respectively.
本文描述了一种气相色谱法，该方法有望作为水中大肠菌群细菌的初步检测方法。通过测定在37℃下产生乙醇所需的培养时间来确定总大肠菌群细菌浓度。粪大肠菌群密度以类似方式在44.5℃下测定。培养基为经滤菌处理的M-9盐，添加1%乳糖、0.1%酪蛋白氨基酸和0.1%酵母提取物。当初始总大肠菌群浓度为每毫升5个或更高，以及粪大肠菌群浓度为每毫升50个或更高时，可获得最佳结果。在这些浓度下的最低检测时间分别为9小时和12小时。
