通过去除玫红酸对M-FC培养基进行改良。
Modification of M-FC medium by eliminating rosolic acid.
作者信息
Presswood W G, Strong D K
出版信息
Appl Environ Microbiol. 1978 Jul;36(1):90-4. doi: 10.1128/aem.36.1.90-94.1978.
Eliminating rosolic acid from M-FC medium improves the MFC procedure by allowing higher fecal coliform colony recoveries with greater ease in counting. Samples of unchlorinated and chlorinated domestic sewage, creek, lake, and river water were analyzed for fecal coliforms by standard procedures. Results of 200 comparisons of fecal coliform counts on M-FC medium without and with rosolic acid showed that higher counts were obtained 71% of the time when rosolic acid was excluded without an overgrowth of background colonies. Results from analyzing chlorinated sewage showed that eliminating rosolic acid improved the recovery of fecal coliform bacteria by 49%. A total of 1,675 blue colonies and 766 nonblue colonies were verified. Of the 1,675 blue colonies, 1,566 were confirmed as fecal coliform bacteria, for a verification of 93.5%. The percent verification of nonblue colonies as noncoliform bacteria was 84.2% (644/766).
从M-FC培养基中去除玫红酸可改进MFC程序，使粪大肠菌群菌落回收率更高，计数也更轻松。采用标准程序对未氯化和已氯化的生活污水、小溪、湖泊及河水样本进行粪大肠菌群分析。对不含玫红酸和含玫红酸的M-FC培养基上的粪大肠菌群计数进行了200次比较，结果表明，在71%的情况下，排除玫红酸时获得的计数更高，且背景菌落没有过度生长。分析氯化污水的结果表明，去除玫红酸可使粪大肠菌群细菌的回收率提高49%。总共验证了1675个蓝色菌落和766个非蓝色菌落。在1675个蓝色菌落中（译注：此处原文有误，应是1566个蓝色菌落），有证实1566个为粪大肠菌群细菌，验证率为93.5%。非蓝色菌落被验证为非大肠菌群细菌的比例为84.2%（644/766）。
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