水中大肠菌群生物检测的辐射测量法。
Radiometric method for the detection of coliform organisms in water.
作者信息
Bachrach U, Bachrach Z
出版信息
Appl Microbiol. 1974 Aug;28(2):169-71. doi: 10.1128/am.28.2.169-171.1974.
Abstract
A new radiometric method for the detection of coliform bacteria in water has been described. The method is based on the release of (14)CO(2) from [(14)C]lactose by bacteria suspended in growth medium and incubated at 37 C. The evolved (14)CO(2) is trapped by hyamine hydroxide and counted in a liquid scintillation spectrometer. The method permits the detection of 1 to 10 organisms within 6 h of incubation. Coliform bacteria suspended in water for several days recover from starvation and may be quantitated by the proposed method. Bacteria from water samples may also be concentrated by filtration through membrane filters and detected by the radiometric assay.
摘要
一种用于检测水中大肠菌群的新放射测量方法已被描述。该方法基于悬浮在生长培养基中并在37℃下培养的细菌从[¹⁴C]乳糖中释放出¹⁴CO₂ 。释放出的¹⁴CO₂ 被氢氧化海胺捕获，并在液体闪烁光谱仪中计数。该方法可在培养6小时内检测出1至10个生物体。悬浮在水中数天的大肠菌群可从饥饿状态中恢复，并可通过所提出的方法进行定量。水样中的细菌也可通过滤膜过滤进行浓缩，并通过放射测量法进行检测。
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