Thomson L A, Hageage G J
Appl Microbiol. 1975 Oct;30(4):616-24. doi: 10.1128/am.30.4.616-624.1975.
A variety of fluorescent excitation light sources were compared using a standard fluorescein solution or a bacterial conjugate with immunofluorescent microscopy. Quantitative data were obtained with microscope photometric apparatus. Both the quantitative data and comparative conjugate titering suggest that the 450-W xenon arc excited significantly more fluorescence than did the more commonly used 250-W mercury arc or the 100-W halogen lamp. The conjugate could be diluted 4 to 32 times more using the 450-W xenon. Additional advantages of 450-W xenon excitation include sufficient energy of wave lengths between 470 to 490 mm, thus permitting narrow-band excitation resulting in less autofluorescence and the ability to perform fluorescent-antibody procedures without the darkening of ambient room light.
使用标准荧光素溶液或细菌结合物，通过免疫荧光显微镜对多种荧光激发光源进行了比较。使用显微镜光度测量仪器获得了定量数据。定量数据和比较结合物滴定均表明，450瓦氙弧激发的荧光明显多于更常用的250瓦汞弧或100瓦卤素灯。使用450瓦氙气时，结合物的稀释倍数可提高4至32倍。450瓦氙气激发的其他优点包括在470至490毫米之间具有足够的波长能量，从而允许进行窄带激发，减少自发荧光，并能够在不使室内环境光变暗的情况下进行荧光抗体程序。
