温度敏感型recB和recC突变的分析

Analysis of temperature-sensitive recB and recC mutations.

作者信息

Kushner S R

出版信息

Basic Life Sci. 1975;5A:301-6. doi: 10.1007/978-1-4684-2895-7_39.

Abstract

The in vivo pleiotropic effects associated with the temperature-sensitive recB270 and recC271 mutations have been correlated with the in vitro behavior of the recBC nucleases coded for by these alleles. The ATP-dependent breakdown of double-stranded DNA is essential for cell viability, radiation repair, and genetic recombination. Temperature sensitivity can be suppressed in vitro and in vivo.

摘要

与温度敏感型recB270和recC271突变相关的体内多效性效应已与这些等位基因编码的recBC核酸酶的体外行为相关联。双链DNA的ATP依赖性分解对于细胞活力、辐射修复和基因重组至关重要。温度敏感性在体外和体内均可被抑制。