Shlaes D M, Barbour S D
J Bacteriol. 1979 Apr;138(1):105-8. doi: 10.1128/jb.138.1.105-108.1979.
An Escherichia coli strain carrying both rec+ and sbcA has been constructed. Repair of ultraviolet light-induced deoxyribonucleic acid damage was examined by measuring survival and thymine-dimer excision in the rec+ sbcA strain as well as rec+ sbcA+ and recB recC sbcA strains. The sbcA mutation restores normal survival in both recB recC uvrB and recB recC uvr+ strains. Excision of thymine-containing dimers does not occur in uvrB mutants, regardless of the rec or sbcA genotype. Survival, after ultraviolet-light damage, of a rec+ sbcA strain is quantitatively similar to rec+ sbcA+ and recB recC sbcA strains.
构建了一株同时携带rec⁺和sbcA的大肠杆菌菌株。通过测量rec⁺ sbcA菌株以及rec⁺ sbcA⁺和recB recC sbcA菌株的存活率和胸腺嘧啶二聚体切除情况,来检测紫外线诱导的脱氧核糖核酸损伤的修复。sbcA突变可恢复recB recC uvrB和recB recC uvr⁺菌株的正常存活率。无论rec或sbcA基因型如何,uvrB突变体中均不会发生含胸腺嘧啶二聚体的切除。rec⁺ sbcA菌株在紫外线损伤后的存活率在数量上与rec⁺ sbcA⁺和recB recC sbcA菌株相似。