温度敏感型recB和recC突变体的体内研究。
In vivo studies of temperature-sensitive recB and recC mutants.
作者信息
Kushner S R
出版信息
J Bacteriol. 1974 Dec;120(3):1213-8. doi: 10.1128/jb.120.3.1213-1218.1974.
Abstract
Some in vivo properties of Escherichia coli K-12 strains carrying recB270 (formerly recBts1) and recC271 (formerly recCts1) mutations have been determined. Single recB270 and recC271 mutants appear normal at 30 C with regard to ultraviolet and mitomycin C sensitivity, recombination proficiency, and viability. At 43 C these strains become sensitive to ultraviolet and mitomycin C, while showing only a slight decrease in recombination proficiency. The viable titers of the single mutants are somewhat reduced at 43 C. Double mutant strains carrying polA1 and recB270 or recC271 are inviable at 43 C. The double mutant strain (recB270 recC271) is sensitive to both UV and mitomycin C at 30 C, but shows only slightly reduced recombination proficiency. At 43 C the strain resembles absolute recB and recC mutants in all respects. In addition, the double mutant strain exhibits a temperature-induced drop in viable titer. The triple mutant polA1 recB270 recC271 is viable at 30 C. Two hypotheses are advanced to explain these results.
摘要
已确定携带recB270（原recBts1）和recC271（原recCts1）突变的大肠杆菌K-12菌株的一些体内特性。单个recB270和recC271突变体在30℃时，在紫外线和丝裂霉素C敏感性、重组能力和活力方面看起来正常。在43℃时，这些菌株对紫外线和丝裂霉素C变得敏感，而重组能力仅略有下降。单个突变体的活菌滴度在43℃时有所降低。携带polA1和recB270或recC271的双突变菌株在43℃时无法存活。双突变菌株（recB270 recC271）在30℃时对紫外线和丝裂霉素C均敏感，但重组能力仅略有下降。在43℃时，该菌株在各方面都类似于绝对recB和recC突变体。此外，双突变菌株的活菌滴度会因温度升高而下降。三突变体polA1 recB270 recC271在30℃时可存活。提出了两种假设来解释这些结果。
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