Griffiths W A, Dutz W
Br J Dermatol. 1975 Jul;93(1):43-5. doi: 10.1111/j.1365-2133.1975.tb06474.x.
A simple and almost painless technique for repeated sampling of dermal infiltrates with a dendritic broach was used in the diagnosis of cutaneous leishmaniasis. This technique can be used to advantage to obtain organisms and cell underneath crusted lesions and to evaluate not only the number of organisms but also the cell pattern at different depths of the lesion. It can be used repeatedly on the same lesions without appreciably disturbing the natural progress of the disease and is therefore well suited to monitor the in vivo effects of therapeutic agents on organisms. Its use in twenty-one cases of cutaneous leishmaniasis and one case of cutaneous leprosy is described.
一种使用树枝状拉刀对皮肤浸润进行重复采样的简单且几乎无痛的技术被用于皮肤利什曼病的诊断。该技术可有效地用于获取痂皮病变下方的病原体和细胞，不仅能评估病原体数量，还能评估病变不同深度的细胞模式。它可以在同一病变上反复使用，而不会明显干扰疾病的自然进程，因此非常适合监测治疗药物对病原体的体内作用。本文描述了该技术在21例皮肤利什曼病和1例皮肤麻风病中的应用。
