Berger R S, Perez-Figaredo R A, Spielvogel R L
J Am Acad Dermatol. 1987 May;16(5 Pt 2):1096-105. doi: 10.1016/s0190-9622(87)70142-x.
A 31-year-old man from Yemen presented with a 6-month history of a nonhealing ulcerated plaque on his right elbow. Initial evaluation included hematoxylin-and-eosin and Giemsa stains of a skin biopsy specimen. These preparations were interpreted as consistent with leishmaniasis, but no organisms were seen. A second biopsy specimen was obtained for culture on special media. A touch preparation demonstrated amastigotes, and cultures demonstrated leishmanial organisms at 2 weeks. The touch preparation as a rapid means of diagnosing leishmaniasis is discussed in detail, and a review of leishmanial disease and treatment is presented.
一名来自也门的31岁男子，其右肘部有一个6个月未愈合的溃疡性斑块。初步评估包括对皮肤活检标本进行苏木精-伊红染色和吉姆萨染色。这些制片结果被解释为与利什曼病相符，但未发现病原体。获取了第二份活检标本在特殊培养基上进行培养。涂片检查发现了无鞭毛体，培养在2周时发现了利什曼原虫。本文详细讨论了涂片检查作为诊断利什曼病的快速方法，并对利什曼病及其治疗进行了综述。
