艰难梭菌基因组中Tn916插入热点的DNA序列以及在整合于同一热点的环境分离株中发现类Tn916元件
DNA sequence of the insertional hot spot of Tn916 in the Clostridium difficile genome and discovery of a Tn916-like element in an environmental isolate integrated in the same hot spot.
作者信息
Wang H, Roberts A P, Mullany P
机构信息
Department of Microbiology, Eastman Dental Institute for Oral Health Care Sciences, University College London, 256 Gray's Inn Road, WC1X 8LD, London, UK.
出版信息
FEMS Microbiol Lett. 2000 Nov 1;192(1):15-20. doi: 10.1111/j.1574-6968.2000.tb09352.x.
Tn916 is a broad host range tetracycline resistance conjugative transposon. In most bacteria, this element enters the bacterial genome at multiple sites. However, in Clostridium difficile, the element has a strong hot spot when introduced by filter mating from Bacillus subtilis. In this work, the DNA sequence of the preferred insertion site (att916) was obtained. An environmental isolate of C. difficile was also discovered which contained an element indistinguishable from Tn916, Tn916CD. Tn916CD was found integrated at att916.
Tn916是一种广宿主范围的四环素抗性接合转座子。在大多数细菌中，该元件可在多个位点进入细菌基因组。然而，在艰难梭菌中，当通过枯草芽孢杆菌滤膜交配引入该元件时，它有一个强烈的热点。在这项研究中，获得了首选插入位点（att916）的DNA序列。还发现了一株艰难梭菌环境分离株，其含有一种与Tn916无法区分的元件，即Tn916CD。发现Tn916CD整合在att916处。
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