A replica-plating method for the identification of Micrococcaceae.

A procedure of replica plating is described whereby all isolated colonies of Micrococcaceae can be identified with relative ease and rapidity. The method is as accurate as the recommended procedure, but permits a more complete and economical analysis of cutaneous flora in large-scale surveys. In this system, Baird-Parker carbohydrate medium was found somewhat superior to standard medium as was incubation at 35 degrees C instead of the customary 30 degrees C. Baird-Parker's broth medium for acetoin production yielded more positive results than did commercial medium, although the reactions were less distinct. However, an agar acetoin test medium was found as good or perhaps even better than Baird-Parker's medium. The classification schemes of Baird-Parker and Bergey's Manual were contrasted in the analysis of data.