Miller J M, Biddle J W, Quenzer V K, McLaughlin J C
Hospital Infections Program, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
J Clin Microbiol. 1993 Dec;31(12):3170-3. doi: 10.1128/jcm.31.12.3170-3173.1993.
The Biolog Identification System (Biolog, Inc., Hayward, Calif.) was challenged at two separate laboratories with 113 coded isolates, including 33 type strains of staphylococci, 5 strains of Micrococcus spp., and 1 strain of Stomatococcus mucilaginosus. Test parameters between the sites were controlled as much as possible. Discrepancies were arbitrated by using conventional biochemicals. Overall accuracies (correct to the species level) upon initial testing were 47.7 and 59.3%, respectively, at the two laboratories. After repeat testing of isolates generating "no identification" responses or errors, the overall accuracies increased to 69.0 and 74.3% at the two sites, respectively, revealing no significant difference in the final results at the two laboratories (78 of 113 versus 84 of 113; P > 0.05). Error rates were 7.1% at one site and 9.7% at the other. The Biolog is not yet accurate enough to serve as a primary method for identifying staphylococci.
生物梅里埃鉴定系统（生物梅里埃公司，加利福尼亚州海沃德）在两个独立实验室接受了113株编码菌株的测试挑战，其中包括33株葡萄球菌标准菌株、5株微球菌属菌株和1株粘液口腔球菌。两个实验室之间的测试参数尽可能保持一致。通过使用传统生化方法对差异进行判定。两个实验室初次测试时的总体准确率（精确到种水平）分别为47.7%和59.3%。在对产生“无法鉴定”结果或错误的菌株进行重复测试后，两个实验室的总体准确率分别提高到了69.0%和74.3%，表明两个实验室的最终结果没有显著差异（113株中，一个实验室为78株，另一个实验室为84株；P>0.05）。一个实验室的错误率为7.1%，另一个为9.7%。生物梅里埃系统用于鉴定葡萄球菌时还不够准确，尚不能作为主要方法。
