Parker J S, Cavell A C, Dolan L, Roberts K, Grierson C S
IACR-Long Ashton Research Station, Department of Agricultural Sciences, University of Bristol, Long Ashton, Bristol, BS41 9AF, United Kingdom.
Plant Cell. 2000 Oct;12(10):1961-74. doi: 10.1105/tpc.12.10.1961.
Root hairs are a major site for the uptake of water and nutrients into plants and form an increasingly important model system for studies of development of higher plants and cell biology. We have identified loss-of-function mutations in eight new genes required for hair growth in Arabidopsis: SHAVEN1 (SHV1), SHV2, and SHV3; CENTIPEDE1 (CEN1), CEN2, and CEN3; BRISTLED1 (BST1); and SUPERCENTIPEDE1 (SCN1). We combined mutations in 79 pairs of genes to determine the stages at which these and six previously known genes contribute to root hair formation. Double mutant phenotypes revealed roles for several genes that could not have been predicted from the single mutant phenotypes. For example, we show that TIP1 and RHD3 are required much earlier in hair formation than previous studies have suggested. We present a genetic model for root hair morphogenesis that defines the roles of each gene, and we suggest hypotheses about functional relationships between genes.
根毛是植物吸收水分和养分的主要部位，并且成为用于研究高等植物发育和细胞生物学的一个越来越重要的模式系统。我们已经在拟南芥中确定了8个根毛生长所需新基因的功能缺失突变体：SHAVEN1 (SHV1)、SHV2和SHV3；CENTIPEDE1 (CEN1)、CEN2和CEN3；BRISTLED1 (BST1)；以及SUPERCENTIPEDE1 (SCN1)。我们将79对基因中的突变体进行组合，以确定这些基因以及6个先前已知的基因在根毛形成过程中发挥作用的阶段。双突变体表型揭示了几个基因的作用，这些作用从单突变体表型中无法预测。例如，我们表明TIP1和RHD3在根毛形成过程中发挥作用的时间比以前的研究所表明的要早得多。我们提出了一个根毛形态发生的遗传模型，该模型定义了每个基因的作用，并且我们提出了关于基因之间功能关系的假设。
