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利用SPAR表型分析平台筛选玉米杂交种的早期干旱胁迫耐受性

Screening corn hybrids for early-stage drought stress tolerance using SPAR phenotyping platform.

作者信息

Lone Ajaz Ahmad, Hussan Shamshir Ul, Jumaa Salah H, Dar Zahoor Ahmad, Reddy K Raja

机构信息

Dryland Agriculture Research Station, Sher e Kashmir University of Agriculture Science & Technology of Kashmir, Srinagar 191132, India.

Department of Plant and Soil Sciences, Mississippi State University, PO Box 9555, Mississippi, 39762, USA.

出版信息

Breed Sci. 2024 Jun;74(3):173-182. doi: 10.1270/jsbbs.23037. Epub 2024 Jul 9.

DOI:10.1270/jsbbs.23037
PMID:39555010
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11561413/
Abstract

An experiment was conducted comprising of six corn hybrids that were subjected to drought and irrigated environment in separate columns in soil-plant-atmosphere-research (SPAR) cubes. The treatments and hybrids in SPAR cubes were replicated four times and a two factorial randomized complete block design (RCBD) was used to analyze the effect of drought on hybrids and their effects on traits. Significant drought × hybrid interactions were observed for most of the parameters. All the traits observed under this study were affected by drought conditions. Root volume (RV) and root shoot ratio (RSR) increased, and number of root tips (NRT), number of root forks (NRF), and number of root crossings (NRC) were drastically reduced under drought conditions. The photosynthetic rate (Phot) declined by 57.96% and electron transport rate (ETR) by 54.60% and was negatively correlated with plant height (PH) and root number (RN) during drought stress. Chlorophyll content (SPAD) showed a non-significant correlation with all the traits. As per results, there were significant differences among corn hybrids for different traits studied under the SPAR setup, which indicates that this setup successfully creates differences in treatments. A cumulative drought stress response index (CDSRI) was worked out. DKC-6581 and N61X-3110 were found to be highly drought tolerant as per our findings.

摘要

进行了一项实验,该实验包括六个玉米杂交种,它们在土壤-植物-大气研究(SPAR)立方体的不同柱中分别处于干旱和灌溉环境。SPAR立方体中的处理和杂交种重复四次,并采用二因素随机完全区组设计(RCBD)来分析干旱对杂交种的影响及其对性状的影响。对于大多数参数,观察到显著的干旱×杂交种相互作用。本研究中观察到的所有性状均受干旱条件影响。在干旱条件下,根体积(RV)和根冠比(RSR)增加,根尖数(NRT)、根叉数(NRF)和根交叉数(NRC)大幅减少。在干旱胁迫期间,光合速率(Phot)下降了57.96%,电子传递速率(ETR)下降了54.60%,并且与株高(PH)和根数(RN)呈负相关。叶绿素含量(SPAD)与所有性状的相关性均不显著。根据结果,在SPAR设置下研究的不同性状的玉米杂交种之间存在显著差异,这表明该设置成功地在处理中创造了差异。计算出了累积干旱胁迫响应指数(CDSRI)。根据我们的研究结果,发现DKC-6581和N61X-3110具有高度耐旱性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6266/11561413/1bd02e049b2d/74_173-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6266/11561413/e4ffe68d871e/74_173-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6266/11561413/d92f6fc078a0/74_173-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6266/11561413/1bd02e049b2d/74_173-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6266/11561413/e4ffe68d871e/74_173-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6266/11561413/d92f6fc078a0/74_173-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6266/11561413/1bd02e049b2d/74_173-g003.jpg

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