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A broad-host-range expression vector series including a Ptac test plasmid and its application in the expression of the dod gene of Serratia marcescens (coding for ribulose-5-phosphate 3-epimerase) in Pseudomonas stutzeri.

作者信息

Graupner S, Wackernagel W

机构信息

Universitaet Oldenburg, AG Genetik, Fachbereich Biologie, Germany.

出版信息

Biomol Eng. 2000 Oct;17(1):11-6. doi: 10.1016/s1389-0344(00)00061-7.

DOI:10.1016/s1389-0344(00)00061-7
PMID:11042472
Abstract

A series of expression vectors for gram-negative bacteria was constructed which combine broad-host-range, inducible expression from the tac promoter and diverse antibiotic resistance determinants. The tac promoter activity and the repression by lacIq can be quantitated with a separate test plasmid in the strain to be studied. The dod gene of Serratia marcescens was expressed in Pseudomonas stutzeri and was shown to code for D-ribulose-5-phosphate 3-epimerase.

摘要

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