• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

用于球形红杆菌和反硝化副球菌的诱导表达质粒。

Inducible-expression plasmid for Rhodobacter sphaeroides and Paracoccus denitrificans.

作者信息

Ind Alice C, Porter Steven L, Brown Mostyn T, Byles Elaine D, de Beyer Jennifer A, Godfrey Scott A, Armitage Judith P

机构信息

Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom.

出版信息

Appl Environ Microbiol. 2009 Oct;75(20):6613-5. doi: 10.1128/AEM.01587-09. Epub 2009 Aug 14.

DOI:10.1128/AEM.01587-09
PMID:19684165
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2765152/
Abstract

We have developed a stable isopropyl-beta-d-thiogalactopyranoside (IPTG)-inducible-expression plasmid, pIND4, which allows graduated levels of protein expression in the alphaproteobacteria Rhodobacter sphaeroides and Paracoccus denitrificans. pIND4 confers kanamycin resistance and combines the stable replicon of pMG160 with the lacI(q) gene from pYanni3 and the lac promoter, P(A1/04/03), from pJBA24.

摘要

我们构建了一种稳定的异丙基-β-D-硫代半乳糖苷（IPTG）诱导表达质粒pIND4，它能在α-变形菌属的球形红细菌和反硝化副球菌中实现不同水平的蛋白质表达。pIND4赋予卡那霉素抗性，并将pMG160的稳定复制子与来自pYanni3的lacI(q)基因以及来自pJBA24的lac启动子P(A1/04/03)相结合。

相似文献

1
Inducible-expression plasmid for Rhodobacter sphaeroides and Paracoccus denitrificans.用于球形红杆菌和反硝化副球菌的诱导表达质粒。
Appl Environ Microbiol. 2009 Oct;75(20):6613-5. doi: 10.1128/AEM.01587-09. Epub 2009 Aug 14.
2
Identification and molecular genetic analysis of multiple loci contributing to high-level tellurite resistance in Rhodobacter sphaeroides 2.4.1.球形红细菌2.4.1中多个导致高水平亚碲酸盐抗性的基因座的鉴定及分子遗传分析
Appl Environ Microbiol. 1997 Dec;63(12):4713-20. doi: 10.1128/aem.63.12.4713-4720.1997.
3
Heterologous expression of correctly assembled methylamine dehydrogenase in Rhodobacter sphaeroides.正确组装的甲胺脱氢酶在球形红杆菌中的异源表达。
J Bacteriol. 1999 Jul;181(14):4216-22. doi: 10.1128/JB.181.14.4216-4222.1999.
4
A powerful hybrid puc operon promoter tightly regulated by both IPTG and low oxygen level.一种受 IPTG 和低氧水平双重严格调控的强杂交 puc 操纵子启动子。
Biochemistry (Mosc). 2010 Apr;75(4):519-2. doi: 10.1134/s0006297910040176.
5
Determination of the Paracoccus denitrificans SOS box.
Microbiology (Reading). 1999 Mar;145 ( Pt 3):577-584. doi: 10.1099/13500872-145-3-577.
6
Thioredoxin is involved in oxygen-regulated formation of the photosynthetic apparatus of Rhodobacter sphaeroides.硫氧还蛋白参与球形红细菌光合装置的氧调节形成。
J Bacteriol. 1999 Jan;181(1):100-6. doi: 10.1128/JB.181.1.100-106.1999.
7
Isolation and molecular characterization of pMG160, a mobilizable cryptic plasmid from Rhodobacter blasticus.来自 Blastobacter 红杆菌的可移动隐蔽质粒 pMG160 的分离与分子特征分析。
Appl Environ Microbiol. 2003 Feb;69(2):725-33. doi: 10.1128/AEM.69.2.725-733.2003.
8
Cloning and characterization of the recA of Paracoccus denitrificans and construction of a recA-deficient mutant.
FEMS Microbiol Lett. 1997 Feb 15;147(2):209-13. doi: 10.1111/j.1574-6968.1997.tb10243.x.
9
Development of inducer-free expression plasmids based on IPTG-inducible promoters for Bacillus subtilis.基于IPTG诱导型启动子的枯草芽孢杆菌无诱导剂表达质粒的构建
Microb Cell Fact. 2017 Jul 25;16(1):130. doi: 10.1186/s12934-017-0747-0.
10
Establishment of a low-dosage-IPTG inducible expression system construction method in Escherichia coli.建立大肠杆菌低剂量 IPTG 诱导表达系统的构建方法。
J Basic Microbiol. 2018 Sep;58(9):806-810. doi: 10.1002/jobm.201800160. Epub 2018 Jul 2.

引用本文的文献

1
A novel engineered strain of Methylorubrum extorquens for methylotrophic production of glycolic acid.一种用于甲基营养型生产乙醇酸的新型工程化甲基营养菌（Methylorubrum extorquens）菌株。
Microb Cell Fact. 2024 Dec 23;23(1):344. doi: 10.1186/s12934-024-02583-y.
2
Tools for genetic engineering and gene expression control in and .在 和 中进行基因工程和基因表达控制的工具。
Appl Environ Microbiol. 2024 Oct 23;90(10):e0034824. doi: 10.1128/aem.00348-24. Epub 2024 Sep 26.
3
Identification and Characterization of an R-M System in DYTN-1 to Improve the Plasmid Conjugation Transfer Efficiency.鉴定和表征 DYTN-1 中的 R-M 系统以提高质粒接合转移效率。
J Microbiol Biotechnol. 2024 Sep 28;34(9):1826-1835. doi: 10.4014/jmb.2402.02041. Epub 2024 Jul 26.
4
Choline degradation in : identification of sources of formaldehyde.胆碱降解物：甲醛来源的鉴定。
J Bacteriol. 2024 Apr 18;206(4):e0008124. doi: 10.1128/jb.00081-24. Epub 2024 Mar 19.
5
A genetic toolbox to empower Paracoccus pantotrophus DSM 2944 as a metabolically versatile SynBio chassis.一个基因工具包，使泛酸营养短小杆菌 DSM 2944 成为具有多功能代谢能力的合成生物学底盘。
Microb Cell Fact. 2024 Feb 15;23(1):53. doi: 10.1186/s12934-024-02325-0.
6
Towards using bacterial microcompartments as a platform for spatial metabolic engineering in the industrially important and metabolically versatile .致力于将细菌微区室用作在具有工业重要性且代谢多样的领域中进行空间代谢工程的平台。
Front Bioeng Biotechnol. 2024 Jan 26;12:1344260. doi: 10.3389/fbioe.2024.1344260. eCollection 2024.
7
Lipid membrane remodeling and metabolic response during isobutanol and ethanol exposure in Zymomonas mobilis.运动发酵单胞菌在异丁醇和乙醇暴露过程中的脂质膜重塑与代谢响应
Biotechnol Biofuels Bioprod. 2024 Jan 28;17(1):14. doi: 10.1186/s13068-023-02450-9.
8
Metabolic engineering of for dual degradation of sulfamethoxazole and ammonia nitrogen.用于磺胺甲恶唑和氨氮双重降解的代谢工程。
Microbiol Spectr. 2023 Sep 21;11(5):e0014623. doi: 10.1128/spectrum.00146-23.
9
Functional Degeneracy in Paracoccus denitrificans Pd1222 Is Coordinated via RamB, Which Links Expression of the Glyoxylate Cycle to Activity of the Ethylmalonyl-CoA Pathway.副球菌 Pd1222 中的功能冗余性通过 RamB 进行协调，RamB 将乙醛酸循环的表达与乙基丙二酰辅酶 A 途径的活性联系起来。
Appl Environ Microbiol. 2023 Jul 26;89(7):e0023823. doi: 10.1128/aem.00238-23. Epub 2023 Jun 15.
10
The role of CenKR in the coordination of cell elongation and division. CenKR 在细胞伸长和分裂协调中的作用。
mBio. 2023 Aug 31;14(4):e0063123. doi: 10.1128/mbio.00631-23. Epub 2023 Jun 7.

本文引用的文献

1
Rhodobacter sphaeroides: complexity in chemotactic signalling.球形红杆菌：趋化信号传导的复杂性
Trends Microbiol. 2008 Jun;16(6):251-60. doi: 10.1016/j.tim.2008.02.006. Epub 2008 Apr 25.
2
Employing Rhodobacter sphaeroides to functionally express and purify human G protein-coupled receptors.利用球形红细菌对人G蛋白偶联受体进行功能表达和纯化。
Biol Chem. 2008 Jan;389(1):69-78. doi: 10.1515/BC.2008.001.
3
Transcriptome dynamics during the transition from anaerobic photosynthesis to aerobic respiration in Rhodobacter sphaeroides 2.4.1.球形红细菌2.4.1从厌氧光合作用向有氧呼吸转变过程中的转录组动态变化
J Bacteriol. 2008 Jan;190(1):286-99. doi: 10.1128/JB.01375-07. Epub 2007 Oct 26.
4
In vivo and in vitro analysis of the Rhodobacter sphaeroides chemotaxis signaling complexes.球形红杆菌趋化信号复合体的体内和体外分析
Methods Enzymol. 2007;423:392-413. doi: 10.1016/S0076-6879(07)23018-6.
5
The CheYs of Rhodobacter sphaeroides.球形红杆菌的CheYs蛋白
J Biol Chem. 2006 Oct 27;281(43):32694-704. doi: 10.1074/jbc.M606016200. Epub 2006 Sep 1.
6
Isolation and molecular characterization of pMG160, a mobilizable cryptic plasmid from Rhodobacter blasticus.来自 Blastobacter 红杆菌的可移动隐蔽质粒 pMG160 的分离与分子特征分析。
Appl Environ Microbiol. 2003 Feb;69(2):725-33. doi: 10.1128/AEM.69.2.725-733.2003.
7
A broad-host-range expression vector series including a Ptac test plasmid and its application in the expression of the dod gene of Serratia marcescens (coding for ribulose-5-phosphate 3-epimerase) in Pseudomonas stutzeri.
Biomol Eng. 2000 Oct;17(1):11-6. doi: 10.1016/s1389-0344(00)00061-7.
8
Fine tuning bacterial chemotaxis: analysis of Rhodobacter sphaeroides behaviour under aerobic and anaerobic conditions by mutation of the major chemotaxis operons and cheY genes.微调细菌趋化性：通过主要趋化性操纵子和cheY基因的突变分析球形红杆菌在有氧和无氧条件下的行为
EMBO J. 2000 Sep 1;19(17):4601-13. doi: 10.1093/emboj/19.17.4601.
9
Thioredoxin is involved in oxygen-regulated formation of the photosynthetic apparatus of Rhodobacter sphaeroides.硫氧还蛋白参与球形红细菌光合装置的氧调节形成。
J Bacteriol. 1999 Jan;181(1):100-6. doi: 10.1128/JB.181.1.100-106.1999.
10
New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria.用于研究细菌中瞬时基因表达的新型绿色荧光蛋白不稳定变体。
Appl Environ Microbiol. 1998 Jun;64(6):2240-6. doi: 10.1128/AEM.64.6.2240-2246.1998.