用于球形红杆菌和反硝化副球菌的诱导表达质粒。
Inducible-expression plasmid for Rhodobacter sphaeroides and Paracoccus denitrificans.
作者信息
Ind Alice C, Porter Steven L, Brown Mostyn T, Byles Elaine D, de Beyer Jennifer A, Godfrey Scott A, Armitage Judith P
机构信息
Department of Biochemistry, University of Oxford, South Parks Road, Oxford OX1 3QU, United Kingdom.
出版信息
Appl Environ Microbiol. 2009 Oct;75(20):6613-5. doi: 10.1128/AEM.01587-09. Epub 2009 Aug 14.
Abstract
We have developed a stable isopropyl-beta-d-thiogalactopyranoside (IPTG)-inducible-expression plasmid, pIND4, which allows graduated levels of protein expression in the alphaproteobacteria Rhodobacter sphaeroides and Paracoccus denitrificans. pIND4 confers kanamycin resistance and combines the stable replicon of pMG160 with the lacI(q) gene from pYanni3 and the lac promoter, P(A1/04/03), from pJBA24.
摘要
我们构建了一种稳定的异丙基-β-D-硫代半乳糖苷（IPTG）诱导表达质粒pIND4，它能在α-变形菌属的球形红细菌和反硝化副球菌中实现不同水平的蛋白质表达。pIND4赋予卡那霉素抗性，并将pMG160的稳定复制子与来自pYanni3的lacI(q)基因以及来自pJBA24的lac启动子P(A1/04/03)相结合。
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2
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Biol Chem. 2008 Jan;389(1):69-78. doi: 10.1515/BC.2008.001.
3
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4
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Methods Enzymol. 2007;423:392-413. doi: 10.1016/S0076-6879(07)23018-6.
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EMBO J. 2000 Sep 1;19(17):4601-13. doi: 10.1093/emboj/19.17.4601.
9
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