海洋细菌 Pseudoalteromonas agarivorans KMM 232（R 型）酸性 O-特异性多糖的结构。

Structure of an acidic O-specific polysaccharide of the marine bacterium Pseudoalteromonas agarivorans KMM 232 (R-form).

机构信息

Pacific Institute of Bioorganic Chemistry, Far East Division, Russian Academy of Sciences, Vladivostok, 690022, Russia.

出版信息

Biochemistry (Mosc). 2010 May;75(5):623-8. doi: 10.1134/s0006297910050123.

PMID:20632942

Abstract

An acidic O-specific polysaccharide containing L-rhamnose, 2-acetamido-2-deoxy-D-galactose, 2,6-dideoxy-2-(N-acetyl-L-threonine)amino-D-galactose, and 2-acetamido-2-deoxy-D-mannuronic acid was obtained by mild acid degradation of the lipopolysaccharide of the marine bacterium Pseudoalteromonas agarivorans KMM 232 (R-form) followed by gel-permeation chromatography. The polysaccharide was subjected to Smith degradation to give a modified polysaccharide with trisaccharide repeating unit containing L-threonine. The initial and modified polysaccharides were studied by sugar analysis and 1H- and 13C-NMR spectroscopy, including COSY, TOCSY, ROESY, and HSQC experiments, and the structure of the branched tetrasaccharide repeating unit of the polysaccharide was established.

摘要

一种酸性 O-特异性多糖，含有 L-鼠李糖、2-乙酰氨基-2-脱氧-D-半乳糖、2,6-二脱氧-2-(N-乙酰-L-苏氨酸)氨基-D-半乳糖和 2-乙酰氨基-2-脱氧-D-甘露糖酸，是通过温和酸降解海洋细菌假交替单胞菌琼脂沃兰斯 KMM 232（R 型）的脂多糖，然后进行凝胶渗透色谱法获得的。多糖经过 Smith 降解，得到含有 L-苏氨酸的三糖重复单元的修饰多糖。初始和修饰多糖通过糖分析和 1H 和 13C-NMR 光谱学（包括 COSY、TOCSY、ROESY 和 HSQC 实验）进行研究，建立了多糖分支四糖重复单元的结构。