Caccamese S, Principato G
Dipartimento di Scienze Chimiche, Università di Catania, Italy.
J Chromatogr A. 2000 Sep 29;893(1):47-54. doi: 10.1016/s0021-9673(00)00688-9.
The four enantiomeric pairs of vincamine group alkaloids were separated by HPLC using Chiralpak AD as chiral stationary phase (CSP) and various n-hexane-2-propanol and n-hexane-ethanol mobile phases. (+)-cis-Vincamine, which is used in pharmaceutical preparations, is eluted much faster than its optical isomer, with separation factors of 2.4 and 3.5, respectively in these mobile phases. Other CSPs gave negative results. A chiral recognition mechanism is proposed and circular dichroism spectra of the individual enantiomers are presented.
以Chiralpak AD为手性固定相（CSP），采用不同的正己烷 - 2 - 丙醇和正己烷 - 乙醇流动相，通过高效液相色谱法（HPLC）分离长春胺类生物碱的四对对映体。用于药物制剂的（+） - 顺式长春胺的洗脱速度比其旋光异构体快得多，在这些流动相中的分离因子分别为2.4和3.5。其他CSPs得到了阴性结果。提出了一种手性识别机制，并给出了各个对映体的圆二色光谱。
