In situ hybridization of chromosome loci.

In situ hybridization is potentially the most powerful technique available for gene mapping, and has already been proved to be useful for these studies. By a combination of the isolation of pure protein-specific mRNA and the synthesis of high specific activity radioactive cDNA to it, this procedure should rapidly increase our knowledg of gene locations, linkages, and possible even heretofore undetectable minor deletions. Studies with in situ hybridization are summarized and some of the questions regarding specificity, grain development, and the like are considered.