Mesmer M Z, Flurer R A
United States Food and Drug Administration, Forensic Chemistry Center, Cincinnati, OH 45237-3097, USA.
J Chromatogr A. 2000 Sep 8;891(2):249-55. doi: 10.1016/s0021-9673(00)00608-7.
This work describes a simple method for the determination and mass-spectrometric confirmation of the indanediones in commercial rodenticides. The sample is sonicated in methanol containing 2% formic acid and analyzed by liquid chromatography-UV detection. Once retention time and UV-Vis spectrum provide tentative identification, mass-spectrometric confirmation is obtained by analyzing a second aliquot by LC and electrospray ionization mass spectrometry (LC-ESI-MS). The extensive fragmentation of the indanedione molecule under MS/MS conditions provides sufficient structural information for positive identification on analyte levels as low as 20 ng on column.
这项工作描述了一种用于测定和质谱确认商用灭鼠剂中茚满二酮的简单方法。将样品在含有2%甲酸的甲醇中超声处理，并通过液相色谱 - 紫外检测进行分析。一旦保留时间和紫外 - 可见光谱提供初步鉴定，通过用液相色谱和电喷雾电离质谱（LC - ESI - MS）分析第二个等分试样来获得质谱确认。茚满二酮分子在MS/MS条件下的广泛碎片化提供了足够的结构信息，以便在柱上低至20 ng的分析物水平上进行阳性鉴定。
