Yang X, Ye R G, Kong Q Y, Yang Q Q, Gao Y, Zhong J H, Wang T
Department of Nephrology, 1st Affiliated Hospital, Sun Yat-sen University of Medical Sciences, Guangzhou, P.R. China.
Adv Perit Dial. 2000;16:213-5.
CD40-CD40 ligand (CD40L) interaction plays an important role in macrophage/monocyte-mediated inflammatory processes by up-regulating cytokine production by macrophages/monocytes and by preventing macrophage apoptosis at the inflammation sites. The present study investigated the possible regulation of CD40L expression in peritonitis during continuous ambulatory peritoneal dialysis (CAPD). We used fluorescence-activated cell sorter (FACS) analysis to detect CD40L expression on macrophages obtained from peritoneal dialysate. Our results showed that CD40L expression on macrophages was significantly increased in a peritonitis group (4.62 +/- 6.54) as compared to a control group (0.76 +/- 0.30, p < 0.01). The CD40L-positive cells were also significantly increased during peritonitis (97.86% +/- 1.67% in the peritonitis group as compared to 73.10% +/- 26.94% in the control group, p < 0.05). After successful treatment, the expression of CD40L was significantly reduced (3.66 +/- 1.12 vs 1.05 +/- 0.02, p < 0.05). We conclude that functionally expressed CD40L on macrophages may take part in acute inflammatory response during peritonitis in CAPD and may play an important role in the local defense against infection in the peritoneal cavity.
CD40与CD40配体（CD40L）的相互作用在巨噬细胞/单核细胞介导的炎症过程中发挥重要作用，它通过上调巨噬细胞/单核细胞的细胞因子产生以及防止炎症部位的巨噬细胞凋亡来实现。本研究调查了持续性非卧床腹膜透析（CAPD）期间腹膜炎中CD40L表达的可能调节情况。我们使用荧光激活细胞分选仪（FACS）分析来检测从腹膜透析液中获得的巨噬细胞上CD40L的表达。我们的结果显示，与对照组（0.76±0.30）相比，腹膜炎组巨噬细胞上的CD40L表达显著增加（4.62±6.54，p<0.01）。腹膜炎期间CD40L阳性细胞也显著增加（腹膜炎组为97.86%±1.67%，对照组为73.10%±26.94%，p<0.05）。成功治疗后，CD40L的表达显著降低（3.66±1.12对1.05±0.02，p<0.05）。我们得出结论，巨噬细胞上功能性表达的CD40L可能参与CAPD腹膜炎期间的急性炎症反应，并可能在腹膜腔局部抗感染防御中发挥重要作用。
