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负责共整合质粒R100-1不相容性的基因位点。

Genetic loci responsible for incompatibility on a co-integrate plasmid, R100-1.

作者信息

Yoshikawa M

出版信息

J Bacteriol. 1975 Dec;124(3):1097-100. doi: 10.1128/jb.124.3.1097-1100.1975.

Abstract

An R plasmid, R100-1, was mapped previously (Yoshikawa, 1974) by transduction from an integratively suppressed Hfr strain to a recipient with a mutation in gene dnaA. By this method various types of transductants of plasmid R100-1 that exist autonomously or in the integrated state were obtained. Seventy-one such transductants were used in the present study to map gene inc, which is responsible for incompatibility. The results obtained can be explained by either of the following: (i) R100-1 has only a single gene or gene cluster (inc) despite previous work suggesting that this plasmid is a co-integrate of two replicons; (ii) R100-1 possesses more than one inc locus located between the repA and tra loci.

摘要

一种R质粒R100 - 1,先前已通过从整合抑制的Hfr菌株转导至基因dnaA发生突变的受体菌进行了图谱绘制(吉川,1974年)。通过这种方法,获得了以自主状态或整合状态存在的质粒R100 - 1的各种类型的转导子。本研究使用了71个这样的转导子来绘制负责不相容性的基因inc的图谱。得到的结果可以用以下两种情况中的任何一种来解释:(i)尽管先前的研究表明该质粒是两个复制子的共整合体,但R100 - 1只有一个单一基因或基因簇(inc);(ii)R100 - 1在repA和tra基因座之间拥有多个inc位点。

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